Plasma-enhanced protein patterning in a microfluidic compartmentalized platform for multi-organs-on-chip: a liver-tumor model.

Autor:	Ferrari E; Politecnico di Milano, Department of Electronics, Information and Bioengineering, Via Golgi 39, Milano 20133, Italy., Ugolini GS; Politecnico di Milano, Department of Electronics, Information and Bioengineering, Via Golgi 39, Milano 20133, Italy., Piutti C; Accelera Srl, Viale Pasteur 10, 20014 Nerviano, MI, Italy., Marzorati S; Accelera Srl, Viale Pasteur 10, 20014 Nerviano, MI, Italy., Rasponi M; Politecnico di Milano, Department of Electronics, Information and Bioengineering, Via Golgi 39, Milano 20133, Italy.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2021 Jun 07; Vol. 16 (4). Date of Electronic Publication: 2021 Jun 07.
DOI:	10.1088/1748-605X/ac0454
Abstrakt:	A microfluidic technique is presented for micropatterning protein domains and cell cultures within permanently bonded organs-on-chip devices. This method is based on the use of polydimethylsiloxane layers coupled with the plasma ablation technique for selective protein removal. We show how this technique can be employed to generate a multi-organ in vitro model directly within a microscale platform suitable for pharmacokinetic-based drug screening. We miniaturized a liver model based on micropatterned co-cultures in dual-compartment microfluidic devices. The cytotoxic effect of liver-metabolized Tegafur on colon cancer cell line was assessed using two microfluidic devices where microgrooves and valves systems are used to model drug diffusion between culture compartments. The platforms can reproduce the metabolism of Tegafur in the liver, thus killing colon cancer cells. The proposed plasma-enhanced microfluidic protein patterning method thus successfully combines the ability to generate precise cell micropatterning with the intrinsic advantages of microfluidics in cell biology. (© 2021 IOP Publishing Ltd.)
Databáze:	MEDLINE
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