Structural Analysis of Strigolactone-Related Gene Products.

Autor:	Andersson I; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden. inger@xray.bmc.uu.se.; Arctic University of Norway, Tromsø, Norway. inger@xray.bmc.uu.se.; Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic. inger@xray.bmc.uu.se., Carlsson GH; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden., Hasse D; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2309, pp. 245-257.
DOI:	10.1007/978-1-0716-1429-7_19
Abstrakt:	Structural knowledge of biological macromolecules is essential for understanding their function and for modifying that function by engineering. Protein crystallography is a powerful method for elucidating molecular structures of proteins, but it is essential that the investigator has a basic knowledge of good practices and of the major pitfalls in the technique. Here we describe issues specific for the case of structural studies of strigolactone (SL) receptor structure and function, and in particular the difficulties associated with capturing complexes of SL receptors with the SL hormone ligand in the crystal.
Databáze:	MEDLINE
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