Spatiotemporal Control Over Multicellular Migration Using Green Light Reversible Cell-Cell Interactions.

Autor: Nzigou Mombo B; Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Waldeyerstraße 15, Münster, 48149, Germany., Bijonowski BM; Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Waldeyerstraße 15, Münster, 48149, Germany., Rasoulinejad S; Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128, Germany., Mueller M; Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128, Germany., Wegner SV; Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Waldeyerstraße 15, Münster, 48149, Germany.; Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128, Germany.
Jazyk: angličtina
Zdroj: Advanced biology [Adv Biol (Weinh)] 2021 May; Vol. 5 (5), pp. e2000199. Date of Electronic Publication: 2021 Jan 14.
DOI: 10.1002/adbi.202000199
Abstrakt: The regulation of cell-cell adhesions in space and time plays a crucial role in cell biology, especially in the coordination of multicellular behavior. Therefore, tools that allow for the modulation of cell-cell interactions with high precision are of great interest to a better understanding of their roles and building tissue-like structures. Herein, the green light-responsive protein CarH is expressed at the plasma membrane of cells as an artificial cell adhesion receptor, so that upon addition of its cofactor vitamin B 12 specific cell-cell interactions form and lead to cell clustering in a concentration-dependent manner. Upon green light illumination, the CarH based cell-cell interactions disassemble and allow for their reversion with high spatiotemporal control. Moreover, these artificial cell-cell interactions impact cell migration, as observed in a wound-healing assay. When the cells interact with each other in the presence of vitamin B 12 in the dark, the cells form on a solid front and migrate collectively; however, under green light illumination, individual cells migrate randomly out of the monolayer. Overall, the possibility of precisely controlling cell-cell interactions and regulating multicellular behavior is a potential pathway to gaining more insight into cell-cell interactions in biological processes.
(© 2021 The Authors. Advanced Biology published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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