Toxicogenomic Profiling of 28 Nanomaterials in Mouse Airways.

Autor: Kinaret PAS; Institute of Biotechnology, Helsinki Institute of Life Science University of Helsinki Helsinki 00790 Finland.; Faculty of Medicine and Health Technology Tampere University Tampere 33720 Finland., Ndika J; Human Microbiome Research Program (HUMI) University of Helsinki Helsinki 00014 Finland., Ilves M; Human Microbiome Research Program (HUMI) University of Helsinki Helsinki 00014 Finland., Wolff H; Finnish Institute of Occupational Health Helsinki 00250 Finland., Vales G; Finnish Institute of Occupational Health Helsinki 00250 Finland., Norppa H; Finnish Institute of Occupational Health Helsinki 00250 Finland., Savolainen K; Finnish Institute of Occupational Health Helsinki 00250 Finland., Skoog T; Department of Biosciences and Nutrition Karolinska Institutet Stockholm 141 83 Sweden., Kere J; Department of Biosciences and Nutrition Karolinska Institutet Stockholm 141 83 Sweden., Moya S; Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA) Donostia-San Sebastián 20014 Spain., Handy RD; School of Biological & Marine Sciences University of Plymouth Plymouth PL4 8AA UK., Karisola P; Human Microbiome Research Program (HUMI) University of Helsinki Helsinki 00014 Finland., Fadeel B; Institute of Environmental Medicine Karolinska Institutet Stockholm 171 77 Sweden., Greco D; Institute of Biotechnology, Helsinki Institute of Life Science University of Helsinki Helsinki 00790 Finland.; Faculty of Medicine and Health Technology Tampere University Tampere 33720 Finland.; BioMediTech Institute Tampere University Tampere 33520 Finland.; Finnish Center for Alternative Methods (FICAM) Tampere 33520 Finland., Alenius H; Human Microbiome Research Program (HUMI) University of Helsinki Helsinki 00014 Finland.; Institute of Environmental Medicine Karolinska Institutet Stockholm 171 77 Sweden.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2021 Mar 08; Vol. 8 (10), pp. 2004588. Date of Electronic Publication: 2021 Mar 08 (Print Publication: 2021).
DOI: 10.1002/advs.202004588
Abstrakt: Toxicogenomics opens novel opportunities for hazard assessment by utilizing computational methods to map molecular events and biological processes. In this study, the transcriptomic and immunopathological changes associated with airway exposure to a total of 28 engineered nanomaterials (ENM) are investigated. The ENM are selected to have different core (Ag, Au, TiO 2 , CuO, nanodiamond, and multiwalled carbon nanotubes) and surface chemistries (COOH, NH 2 , or polyethylene glycosylation (PEG)). Additionally, ENM with variations in either size (Au) or shape (TiO 2 ) are included. Mice are exposed to 10 µg of ENM by oropharyngeal aspiration for 4 consecutive days, followed by extensive histological/cytological analyses and transcriptomic characterization of lung tissue. The results demonstrate that transcriptomic alterations are correlated with the inflammatory cell infiltrate in the lungs. Surface modification has varying effects on the airways with amination rendering the strongest inflammatory response, while PEGylation suppresses toxicity. However, toxicological responses are also dependent on ENM core chemistry. In addition to ENM-specific transcriptional changes, a subset of 50 shared differentially expressed genes is also highlighted that cluster these ENM according to their toxicity. This study provides the largest in vivo data set currently available and as such provides valuable information to be utilized in developing predictive models for ENM toxicity.
Competing Interests: The authors declare no conflict of interest.
(© 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje