Kinetics of Influenza A/BANGKOK/1/1979(Н3N2) Virus Thermal Inactivation in the Presence of Polyallylamine.

Autor: Kontarov NA; Sechenov First Moscow State Medical University (Sechenov University), Ministry of Healthcare of the Russian Federation, 119991 Moscow, Russia.; Mechnikov Research Institute of Vaccines and Sera, 115088 Moscow, Russia., Dolgova EI; Mechnikov Research Institute of Vaccines and Sera, 115088 Moscow, Russia., Pogarskaya IV; Mechnikov Research Institute of Vaccines and Sera, 115088 Moscow, Russia., Kontarova EO; Federal Research and Clinical Center, Federal Medical and Biological Agency, 115682 Moscow, Russia., Yuminova NV; Mechnikov Research Institute of Vaccines and Sera, 115088 Moscow, Russia.
Jazyk: angličtina
Zdroj: Moscow University biological sciences bulletin [Moscow Univ Biol Sci Bull] 2021; Vol. 76 (1), pp. 34-38. Date of Electronic Publication: 2021 May 18.
DOI: 10.3103/S0096392521010028
Abstrakt: Polyelectrolytes currently play an increasingly important role in antivirus therapy. Antiviral activity towards influenza virus, measles virus, herpes simplex virus type 1, and cytomegalovirus was demonstrated for the 6000 Da polyelectrolyte polyallylamine. A nontoxic polyallylamine concentration of 30 µM at which the compound retains its antiviral effect towards measles and influenza viruses but lacks any toxic effect on human cells was previously determined. It is well known, at the same time, that simultaneous virus exposure to physical environmental factors and chemical substances causes a more significant decrease in virus infectivity. Temperature is among these physical factors since thermal exposure causes virus inactivation. Analysis of virus thermal inactivation parameters is of high practical importance when it comes to the development of vaccines against influenza virus and to the study of how virus particles infectivity decreases on various surfaces. In this view, the study of kinetic and thermodynamical characteristics of influenza virus thermal inactivation in the presence of the antiviral preparation polyallylanime is of particular interest. The paper reports that thermal inactivation of influenza virus in the temperature range of 38-60°C in the presence of polyallylamine follows the first-order reaction kinetics. Thermodynamic parameters of influenza virus thermal inactivation evidence that influenza virus surface proteins are involved in the inactivation process as a result of their interaction with polyallylamine. The obtained results show that polyallylamine may be used to accelerate thermal inactivation of the influenza virus.
Competing Interests: COMPLIANCE WITH ETHICAL STANDARDSThe authors declare that they have no conflicts of interest. No experimentation involving animals or humans was carried out by any of the authors.
(© Allerton Press, Inc. 2021, ISSN 0096-3925, Moscow University Biological Sciences Bulletin, 2021, Vol. 76, No. 1, pp. 34–38. © Allerton Press, Inc., 2021.Russian Text © The Author(s), 2021, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2021, Vol. 76, No. 1, pp. 41–46.)
Databáze: MEDLINE
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