Localization and characterization of human papillomavirus-16 in oral squamous cell carcinoma.

Autor: Saleh W; Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA.; Oral Medicine and Periodontology Department, Faculty of Dentistry, Mansoura University, Mansoura, FL, USA., Cha S; Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA., Banasser A; Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA., Fitzpatrick SG; Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA., Bhattacharyya I; Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA., Youssef JM; Oral Medicine and Periodontology Department, Faculty of Dentistry, Mansoura University, Mansoura, FL, USA., Anees MM; Oral Medicine and Periodontology Department, Faculty of Dentistry, Mansoura University, Mansoura, FL, USA., Elzahaby IA; Department of Surgical Oncology, Mansoura Oncology Center, Mansoura University, Mansoura, FL, USA., Katz J; Oral Diagnostic Sciences Department, College of Dentistry, University of Florida, Gainesville, FL, USA.
Jazyk: angličtina
Zdroj: Oral diseases [Oral Dis] 2023 Mar; Vol. 29 (2), pp. 436-444. Date of Electronic Publication: 2021 Jun 21.
DOI: 10.1111/odi.13920
Abstrakt: Objectives: The role of Human papillomavirus (HPV) in the oral squamous cell carcinoma (OSCC) has not been completely elucidated. The purpose of the present study was to investigate the prevalence and localization of HPV-16 virus in OSCC and to correlate HPV-16 positivity and p16INK4A expression with the clinical and pathological features of OSCC.
Methods: The archives of Oral Pathology at the University of Florida, College of Dentistry were accessed for demographic, clinical, histopathological data, and slides of 114 OSCC patients. HPV-16 positivity of OSCC was evaluated by p16INK4A immunohistochemistry (IHC) and HPV-16 E6/E7mRNA by in situ hybridization (ISH).
Results: Out of 114 consecutive pathological slides of OSCC, 16 samples (14%) showed positivity for p16INK4A by IHC and 14 samples (12%) were positive for HPV-16 E6/E7mRNA ISH and the Positivity showed a significant correlation with the patients' age, alcohol consumption, and the degree of OSSC differentiation. The hard palate showed the highest positivity of p16INK4A IHC and HPV-16 mRNA ISH (38%, 36% respectively).
Conclusion: HPV-16 is a significant factor in oral carcinogenesis. We recommend using p16INK4A as a surrogate marker for HPV detection in OSCC, which can be complemented by RNA ISH for the identification of HPV subtypes.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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