Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution.

Autor:	Berardi M; LaserLab, Department of Physics and Astronomy, VU University, De Boelelaan 1081, Amsterdam, The Netherlands. massimiliano.berardi@optics11.com.; Optics11, De Boelelaan 1081, Amsterdam, The Netherlands. massimiliano.berardi@optics11.com., Bielawski K; Optics11, De Boelelaan 1081, Amsterdam, The Netherlands., Rijnveld N; Optics11, De Boelelaan 1081, Amsterdam, The Netherlands., Gruca G; Optics11, De Boelelaan 1081, Amsterdam, The Netherlands., Aardema H; Department of Farm Animal Health, Faculty of Veterinary medicine, Utrecht University, Yalelaan 7, Utrecht, The Netherlands., van Tol L; Department of Farm Animal Health, Faculty of Veterinary medicine, Utrecht University, Yalelaan 7, Utrecht, The Netherlands., Wuite G; LaserLab, Department of Physics and Astronomy, VU University, De Boelelaan 1081, Amsterdam, The Netherlands., Akca BI; LaserLab, Department of Physics and Astronomy, VU University, De Boelelaan 1081, Amsterdam, The Netherlands. b.i.avci@vu.nl.
Jazyk:	angličtina
Zdroj:	Communications biology [Commun Biol] 2021 May 21; Vol. 4 (1), pp. 610. Date of Electronic Publication: 2021 May 21.
DOI:	10.1038/s42003-021-02121-1
Abstrakt:	Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.
Databáze:	MEDLINE
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