Unraveling Ascaris suum experimental infection in humans.

Autor: Silva TED; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Barbosa FS; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Magalhães LMD; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gazzinelli-Guimarães PH; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; National Institutes of Health, National Institute of Allergy and Infectious Disease, Bethesda, MD, USA., Dos Santos AC; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Nogueira DS; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Resende NM; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Amorim CC; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gazzinelli-Guimarães AC; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Viana AG; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Geiger SM; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Bartholomeu DC; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Fujiwara RT; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Bueno LL; Parasitology Department, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: llbueno@icb.ufmg.br.
Jazyk: angličtina
Zdroj: Microbes and infection [Microbes Infect] 2021 Sep-Oct; Vol. 23 (8), pp. 104836. Date of Electronic Publication: 2021 May 19.
DOI: 10.1016/j.micinf.2021.104836
Abstrakt: Ascaris lumbricoides and Ascaris suum are two closely related parasites that infect humans and pigs. The zoonotic potential of A. suum has been a matter of debate for decades. Here we sought to investigate the potential human infection by A. suum and its immunological alterations. We orally infected five healthy human subjects with eggs embraced by A. suum. The infection was monitored for symptoms and possible respiratory changes, by an interdisciplinary health team. Parasitological, hematological analyses, serum immunoglobulin, cytokine profiles, and gene expression were evaluated during the infection. Our results show that A. suum is able to infect and complete the cycle in humans causing A. lumbricoides similar symptoms, including, cough, headache, diarrhea, respiratory discomfort and chest x-ray alterations coinciding with larvae migration in the lungs. We also observed activation of the immune system with production of IgM and IgG and a Th2/Th17 response with downregulation of genes related to Th1 and apoptosis. PCA (Principal componts analysis) show that infection with A. suum leads to a change in the immune landscape of the human host. Our data reinforce the zoonotic capacity of A. suum and bring a new perspective on the understanding of the immune response against this parasite.
Competing Interests: Declaration of competing interest The authors declare that they have no competing interests.
(Copyright © 2021. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
Externí odkaz: