FASCIN regulates actin assembly for spindle movement and polar body extrusion in mouse oocyte meiosis.

Autor: Hu LL; Reproductive Medicine Center, the Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.; School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, China., Pan MH; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China., Yang FL; School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, China., Zong ZA; School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, China., Tang F; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China., Pan ZN; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China., Lu X; College of Basic Medical Sciences, Zunyi Medical University, Zunyi, China., Ren YP; College of Basic Medical Sciences, Zunyi Medical University, Zunyi, China., Wang JL; School of Laboratory Medicine, Youjiang Medical University for Nationalities, Baise, China., Sun SC; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Jazyk: angličtina
Zdroj: Journal of cellular physiology [J Cell Physiol] 2021 Nov; Vol. 236 (11), pp. 7725-7733. Date of Electronic Publication: 2021 May 21.
DOI: 10.1002/jcp.30443
Abstrakt: During mouse oocyte meiotic maturation, actin filaments play multiple roles in meiosis such as spindle migration and cytokinesis. FASCIN is shown to be an actin-binding and bundling protein, making actin filaments tightly packed and parallel-aligned, and FASCIN is involved in several cellular processes like adhesion and migration. FASCIN is also a potential prognostic biomarker and therapeutic target for the treatment of metastatic disease. However, little is known about the functions of FASCIN in oocyte meiosis. In the present study, we knocked down the expression of FASCIN, and our results showed that FASCIN was essential for oocyte maturation. FASCIN was all expressed in the different stages of oocyte meiosis, and it mainly localized at the cortex of oocytes from the GV stage to the MII stage and showed a similar localization pattern with actin and DAAM1. Depletion of FASCIN affected the extrusion of the first polar body, and we also observed that some oocytes extruded from the large polar bodies. This might have resulted from the defects of actin assembly, which further affected the meiotic spindle positioning. In addition, we showed that inhibition of PKC activity decreased FASCIN expression, indicating that FASCIN might be regulated by PKC. Taken together, our results provided evidence for the important role of FASCIN on actin filaments for spindle migration and polar body extrusion in mouse oocyte meiosis.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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