| Autor: |
Dholpuria S; Department of Life Science, Sharda University, Greater Noida, India. sunny.dholpuria@sharda.ac.in., Kumar S; Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India., Kumar M; Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India., Sarwalia P; Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India., Kumar R; Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India., Datta TK; Animal Biotechnology Centre, National Dairy Research Institute, Karnal, Haryana, India. tirtha.datta@icar.gov.in. |
| Abstrakt: |
Studying the maternal oocyte-specific genes, in farm animals is a significant step towards delineating the underlying mechanisms that regulate oocyte quality, early embryonic development and survival. With the creation of buffalo oocyte-specific subtracted cDNA library, it has raised new questions which need to be answered. The present study has characterized one of the ESTs selected from the library and highlighted its importance in the oocyte quality. The selected EST was made full length by RLM-RACE and four transcript variants were identified. Bioinformatics analysis indicated the novelty of full-length transcript along with conserved intergenic nature. The largest transcript was identified as long intergenic noncoding RNA based upon coding potential calculator output. The expression analysis at different hours of oocyte maturation showed a significant variation in developmentally competent oocytes to that of incompetent ones. Along with this, the transcript was also found to have protein binding ability which was confirmed by RNA electrophoretic mobility shift assay. The protein used in the experiment was isolated from oocyte and cumulus cells via sonication. A novel lincRNA has been reported here that might have an important role in maturation of oocytes, inferred from its relative gene expression study and protein binding characteristics. |
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