SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity.

Autor:	Joyce MG; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; Lead contact., Chen WH; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Sankhala RS; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Hajduczki A; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Thomas PV; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Choe M; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Chang W; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Peterson CE; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Martinez E; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA., Morrison EB; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Smith C; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MD, USA., Ahmed A; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Wieczorek L; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Anderson A; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Chen RE; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA., Case JB; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA., Li Y; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Oertel T; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Rosado L; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Ganesh A; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Whalen C; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Carmen JM; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Mendez-Rivera L; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Karch C; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Gohain N; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Villar Z; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., McCurdy D; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Beck Z; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Kim J; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Shrivastava S; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Jobe O; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Dussupt V; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Molnar S; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Tran U; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Kannadka CB; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Zemil M; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Khanh H; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MD, USA., Wu W; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MD, USA., Cole MA; Trudeau Institute, Inc., Saranac Lake, NY, USA., Duso DK; Trudeau Institute, Inc., Saranac Lake, NY, USA., Kummer LW; Trudeau Institute, Inc., Saranac Lake, NY, USA., Lang TJ; Trudeau Institute, Inc., Saranac Lake, NY, USA., Muncil SE; Trudeau Institute, Inc., Saranac Lake, NY, USA., Currier JR; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Krebs SJ; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Polonis VR; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Rajan S; Antibody Discovery and Protein Engineering (ADPE), BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, USA., McTamney PM; Microbial Sciences, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, USA., Esser MT; Microbial Sciences, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, USA., Reiley WW; Trudeau Institute, Inc., Saranac Lake, NY, USA., Rolland M; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD, USA.; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., de Val N; Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, USA.; Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Leidos Biomedical Research Inc., Frederick, MD, USA., Diamond MS; Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Pathology & Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA; Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA; The Andrew M. and Jane M. Bursky Center for Human Immunology & Immunotherapy Programs, Washington University School of Medicine, St. Louis, MO 63110, USA., Gromowski GD; Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Matyas GR; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Rao M; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Michael NL; Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USA., Modjarrad K; Emerging Infectious Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2021 May 10. Date of Electronic Publication: 2021 May 10.
DOI:	10.1101/2021.05.09.443331
Abstrakt:	The need for SARS-CoV-2 next-generation vaccines has been highlighted by the rise of variants of concern (VoC) and the long-term threat of other coronaviruses. Here, we designed and characterized four categories of engineered nanoparticle immunogens that recapitulate the structural and antigenic properties of prefusion Spike (S), S1 and RBD. These immunogens induced robust S-binding, ACE2-inhibition, and authentic and pseudovirus neutralizing antibodies against SARS-CoV-2 in mice. A Spike-ferritin nanoparticle (SpFN) vaccine elicited neutralizing titers more than 20-fold higher than convalescent donor serum, following a single immunization, while RBD-Ferritin nanoparticle (RFN) immunogens elicited similar responses after two immunizations. Passive transfer of IgG purified from SpFN- or RFN-immunized mice protected K18-hACE2 transgenic mice from a lethal SARS-CoV-2 virus challenge. Furthermore, SpFN- and RFN-immunization elicited ACE2 blocking activity and neutralizing ID50 antibody titers >2,000 against SARS-CoV-1, along with high magnitude neutralizing titers against major VoC. These results provide design strategies for pan-coronavirus vaccine development. Highlights: Iterative structure-based design of four Spike-domain Ferritin nanoparticle classes of immunogensSpFN-ALFQ and RFN-ALFQ immunization elicits potent neutralizing activity against SARS-CoV-2, variants of concern, and SARS-CoV-1Passively transferred IgG from immunized C57BL/6 mice protects K18-hACE2 mice from lethal SARS-CoV-2 challenge.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu