HLA-DR-Positive NK Cells Expand in Response to Mycobacterium Tuberculosis Antigens and Mediate Mycobacteria-Induced T Cell Activation.

Autor:	Kust SA; Laboratory of Cell Interactions, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Science, Moscow, Russia., Streltsova MA; Laboratory of Cell Interactions, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Science, Moscow, Russia., Panteleev AV; Laboratory of Biotechnology, Central Tuberculosis Research Institute, Moscow, Russia., Karpina NL; Diagnostic Outpatient Department, Central Tuberculosis Research Institute, Moscow, Russia., Lyadova IV; Laboratory of Biotechnology, Central Tuberculosis Research Institute, Moscow, Russia.; Laboratory of Cellular and Molecular Basis of Histogenesis, Koltzov Institute of Developmental Biology of the Russian Academy of Sciences, Moscow, Russia., Sapozhnikov AM; Laboratory of Cell Interactions, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Science, Moscow, Russia., Kovalenko EI; Laboratory of Cell Interactions, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Science, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2021 May 03; Vol. 12, pp. 662128. Date of Electronic Publication: 2021 May 03 (Print Publication: 2021).
DOI:	10.3389/fimmu.2021.662128
Abstrakt:	NK cells play an important role in the control of tuberculosis infection: they are not only able to kill the infected cells, but also control the activity of macrophages and development of the adaptive immune response. Still, there is little information on the role of specific NK cell subsets in this network. In this study, we focused on the mycobacteria-driven responses of the NK cells expressing HLA-DR - a type of MHC class II. We have revealed that this subset is increased in the peripheral blood of patients with primary diagnosed tuberculosis, and expands in response to in vitro stimulation with ultrasonically destroyed Mycobacterium tuberculosis cells (sonicate). The expanded HLA-DR + NK cells had less differentiated phenotype, higher proliferative activity and increased expression of NKp30 and NKp46 receptors. HLA-DR + CD56 dim NK cells showed higher IFNγ production and degranulation level than the respective HLA-DR - NK cells in response to both 24 h and 7 day stimulation with sonicate, while HLA-DR + CD56 bright NK cells mostly demonstarted similar high responsiveness to the same stimulating conditions as their HLA-DR - CD56 bright counterparts. After preliminary incubation with destroyed mycobacteria, cytokine-activated HLA-DR-expressing NK cells were able to mediate mycobacteria-induced and HLA-DR-dependent cytokine production in autologous CD4 + T cells. Thus, functionally active HLA-DR + cells seem to be one of the NK cell subsets providing an important link to the adaptive immunity. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Kust, Streltsova, Panteleev, Karpina, Lyadova, Sapozhnikov and Kovalenko.)
Databáze:	MEDLINE
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