Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system.

Autor: Horváthová L; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Žárský V; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Pánek T; Faculty of Science, Department of Biology and Ecology, University of Ostrava, Ostrava, Czech Republic.; Faculty of Science, Department of Zoology, Charles University, Prague 2, Czech Republic., Derelle R; School of Biosciences, University of Birmingham, Edgbaston, UK., Pyrih J; Laboratory of Molecular & Evolutionary Parasitology, RAPID group, School of Biosciences, University of Kent, Canterbury, UK.; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic., Motyčková A; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Klápšťová V; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Vinopalová M; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Marková L; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Voleman L; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Klimeš V; Faculty of Science, Department of Biology and Ecology, University of Ostrava, Ostrava, Czech Republic., Petrů M; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Vaitová Z; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic., Čepička I; Faculty of Science, Department of Zoology, Charles University, Prague 2, Czech Republic., Hryzáková K; Faculty of Science, Department of Genetics and Microbiology, Charles University, Prague 2, Czech Republic., Harant K; Faculty of Science, Proteomic core facility, Charles University, BIOCEV, Vestec, Czech Republic., Gray MW; Department of Biochemistry and Molecular Biology and Centre for Comparative Genomics and Evolutionary Bioinformatics, Dalhousie University, Halifax, NS, Canada., Chami M; Center for Cellular Imaging and NanoAnalytics, University of Basel, Basel, Switzerland., Guilvout I; Biochemistry of Macromolecular Interactions Unit, Department of Structural Biology and Chemistry, Institut Pasteur, CNRS UMR3528, Paris, France., Francetic O; Biochemistry of Macromolecular Interactions Unit, Department of Structural Biology and Chemistry, Institut Pasteur, CNRS UMR3528, Paris, France., Franz Lang B; Robert Cedergren Centre for Bioinformatics and Genomics, Département de Biochimie, Université de Montréal, Montreal, QC, Canada., Vlček Č; Institute of Molecular Genetics, Czech Academy of Sciences, Prague 4, Czech Republic., Tsaousis AD; Laboratory of Molecular & Evolutionary Parasitology, RAPID group, School of Biosciences, University of Kent, Canterbury, UK., Eliáš M; Faculty of Science, Department of Biology and Ecology, University of Ostrava, Ostrava, Czech Republic. marek.elias@osu.cz., Doležal P; Faculty of Science, Department of Parasitology, Charles University, BIOCEV, Vestec, Czech Republic. pavel.dolezal@natur.cuni.cz.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 May 19; Vol. 12 (1), pp. 2947. Date of Electronic Publication: 2021 May 19.
DOI: 10.1038/s41467-021-23046-7
Abstrakt: The type 2 secretion system (T2SS) is present in some Gram-negative eubacteria and used to secrete proteins across the outer membrane. Here we report that certain representative heteroloboseans, jakobids, malawimonads and hemimastigotes unexpectedly possess homologues of core T2SS components. We show that at least some of them are present in mitochondria, and their behaviour in biochemical assays is consistent with the presence of a mitochondrial T2SS-derived system (miT2SS). We additionally identified 23 protein families co-occurring with miT2SS in eukaryotes. Seven of these proteins could be directly linked to the core miT2SS by functional data and/or sequence features, whereas others may represent different parts of a broader functional pathway, possibly also involving the peroxisome. Its distribution in eukaryotes and phylogenetic evidence together indicate that the miT2SS-centred pathway is an ancestral eukaryotic trait. Our findings thus have direct implications for the functional properties of the early mitochondrion.
Databáze: MEDLINE
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