| Autor: |
Farrag HMM; Parasitology Department, Faculty of Medicine, Assiut University, 71526, Assiut, Egypt.; Faculty of Applied Medical Science, Shaqra University, Kingdom of Saudi Arabia., Yones DA; Parasitology Department, Faculty of Medicine, Assiut University, 71526, Assiut, Egypt., Hassanin ESA; Clinical Pathologist, Ministry of Health, 71515, Assiut, Egypt., Ibraheim ZZ; Pharmacognosy Department, Faculty of Pharmacy, Assiut University, 71526, Assiut, Egypt., Huseein EAEHM; Parasitology Department, Faculty of Medicine, Assiut University, 71526, Assiut, Egypt. |
| Abstrakt: |
Trypanosomosis is a worldwide disease that affects human and livestock populations with limited availability and high cost of trypanocides. The study aims to evaluate the possible in vitro and in vivo anti-trypanosomal activity of Thymus vulgaris (thyme), Mentha piperita (mint) and Elettaria cardamomum (cardamom) aqueous extracts against Trypanosoma evansi in experimentally infected rats using Intropar as a reference drug. The crude extracts of the selected plants were used in three concentrations (2500, 2000, and 1000 μg/ml). The in vitro trypanocidal activities were assessed regarding parasite motility, count, and infectivity. The in vivo susceptibility of T. evansi was evaluated by assessing the level of parasitemia in the experimental rats. The packed cell volume (PCV) was also monitored. Both the in vitro and in vivo experiments showed trypanocidal activity, of all the tested extracts, higher than that of Intropar. The in vitro trypanocidal effects were dose-dependent and represented by a significant reduction of the parasite count together with immobilizing effect within 3 hours incubation period, compared to the negative and positive controls (p< 0.05). The in vivo trypanocidal effects of the different concentrations of all the tested extracts were represented by the significantly lowered levels of parasitemia, compared to the negative control (NC) group with varying degrees; in a dose-dependent manner concerning the time. They exhibited also a significantly higher level in PCV recovery compared to the NC group (p < 0.05). This study initially confirmed the potent in vitro and in vivo trypanocidal effect of the three extracts, with a potentially promising future for the treatment of trypanosomosis. |
