| Autor: |
Huynh GT; Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.; ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Node, Clayton, VIC 3800, Australia., Henderson EC; Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.; ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Node, Clayton, VIC 3800, Australia., Frith JE; Monash Institute of Medical Engineering, Monash University, Clayton, VIC 3800, Australia.; Department of Material Science and Engineering, Monash University, Clayton, VIC 3800, Australia.; ARC Training Centre for Cell and Tissue Engineering Technologies, Monash University, Clayton, VIC 3800, Australia., Meagher L; Department of Material Science and Engineering, Monash University, Clayton, VIC 3800, Australia.; ARC Training Centre for Cell and Tissue Engineering Technologies, Monash University, Clayton, VIC 3800, Australia., Corrie SR; Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia.; ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Node, Clayton, VIC 3800, Australia.; ARC Training Centre for Cell and Tissue Engineering Technologies, Monash University, Clayton, VIC 3800, Australia. |
| Abstrakt: |
Long-term stability and function are key challenges for optical nanosensors operating in complex biological environments. While much focus is rightly placed on issues related to specificity, sensitivity, reversibility, and response time, many nanosensors are not capable of transducing accurate results over prolonged time periods. Sensors could fail over time due to the degradation of scaffold material, degradation of signaling dyes and components, or a combination of both. It is critical to investigate how such degradative processes affect sensor output, as the consequences could be severe. Herein, we used fluorescent core-shell organosilica pH nanosensors as a model system, incubating them in a range of common aqueous solutions over time at different temperatures, and then searched for changes in fluorescence signal, particle size, and evidence of silica degradation. We found that these ratiometric nanosensors produced stable optical signals after aging for 30 days at 37 °C in standard saline buffers with and without 10% fetal bovine serum, and without any evidence of material degradation. Next, we evaluated their performance as real-time pH nanosensors in bacterial suspension cultures, observing a close agreement with a pH electrode for control nanosensors, yet observing obvious deviations in signal based on the aging conditions. The results show that while the organosilica scaffold does not degrade appreciably over time, careful selection of dyes and further systematic investigations into the effects of salt and protein levels are required to realize long-term stable nanosensors. |
