Onset of taste bud cell renewal starts at birth and coincides with a shift in SHH function.

Autor:	Golden EJ; Department of Cell & Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, United States.; The Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, Aurora, United States., Larson ED; The Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, Aurora, United States.; Department of Otolaryngology, University of Colorado Anschutz Medical Campus, Aurora, United States., Shechtman LA; Department of Cell & Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, United States.; The Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, Aurora, United States., Trahan GD; Department of Pediatrics, Section of Hematology, Oncology, and Bone Marrow Transplant, University of Colorado Anschutz Medical Campus, Aurora, United States., Gaillard D; Department of Cell & Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, United States.; The Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, Aurora, United States., Fellin TJ; Department of Cell & Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, United States.; The Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, Aurora, United States., Scott JK; Department of Cell & Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, United States.; The Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, Aurora, United States., Jones KL; Department of Pediatrics, Section of Hematology, Oncology, and Bone Marrow Transplant, University of Colorado Anschutz Medical Campus, Aurora, United States., Barlow LA; Department of Cell & Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, United States.; The Rocky Mountain Taste and Smell Center, University of Colorado Anschutz Medical Campus, Aurora, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 May 19; Vol. 10. Date of Electronic Publication: 2021 May 19.
DOI:	10.7554/eLife.64013
Abstrakt:	Embryonic taste bud primordia are specified as taste placodes on the tongue surface and differentiate into the first taste receptor cells (TRCs) at birth. Throughout adult life, TRCs are continually regenerated from epithelial progenitors. Sonic hedgehog (SHH) signaling regulates TRC development and renewal, repressing taste fate embryonically, but promoting TRC differentiation in adults. Here, using mouse models, we show TRC renewal initiates at birth and coincides with onset of SHHs pro-taste function. Using transcriptional profiling to explore molecular regulators of renewal, we identified Foxa1 and Foxa2 as potential SHH target genes in lingual progenitors at birth and show that SHH overexpression in vivo alters FoxA1 and FoxA2 expression relevant to taste buds. We further bioinformatically identify genes relevant to cell adhesion and cell locomotion likely regulated by FOXA1;FOXA2 and show that expression of these candidates is also altered by forced SHH expression. We present a new model where SHH promotes TRC differentiation by regulating changes in epithelial cell adhesion and migration. Competing Interests: EG, EL, LS, GT, DG, TF, JS, KJ, LB No competing interests declared (© 2021, Golden et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu