Alpha Lipoic Acid Supplementation Improves Ovarian Tissue Vitrification Outcome: An Alternative to Preserve the Ovarian Function of Morada Nova Ewe.

Autor: Ñaupas LVS; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará (UECE), Fortaleza, CE, Brazil., Brito DCC; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará (UECE), Fortaleza, CE, Brazil., de Souza SS; Laboratory of Image Diagnosis Applied to Animal Reproduction, Faculty of Veterinary Medicine, UECE, Fortaleza, CE, Brazil., Brandão FAS; Laboratory of Image Diagnosis Applied to Animal Reproduction, Faculty of Veterinary Medicine, UECE, Fortaleza, CE, Brazil., da Silva RF; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará (UECE), Fortaleza, CE, Brazil., da Silva Raposo R; Nucleus of Experimental Biology, University of Fortaleza, Fortaleza, CE, Brazil., de Oliveira Monteiro Moreira AC; Nucleus of Experimental Biology, University of Fortaleza, Fortaleza, CE, Brazil., Araújo AA; State University of Ceará, Fortaleza, CE, Brazil., Alves BG; Animal Bioscience Postgraduate Program, Federal University of Goiás, Jataí, GO, Brazil., Guedes MIF; Laboratory of Biotechnology and Molecular Biology, UECE, Fortaleza, CE, Brazil., Silva JYG; Laboratory of Biotechnology and Molecular Biology, UECE, Fortaleza, CE, Brazil., Cordova A; Department of Biomedical Sciences, University of Guelph, Guelph, ON, Canada., Figueiredo JR; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará (UECE), Fortaleza, CE, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará (UECE), Fortaleza, CE, Brazil. aprrodriguespapers@gmail.com.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2021 Nov; Vol. 28 (11), pp. 3109-3122. Date of Electronic Publication: 2021 May 18.
DOI: 10.1007/s43032-021-00593-4
Abstrakt: This study evaluated the effect of adding alpha lipoic acid (ALA) to the vitrification solution of sheep ovarian tissue on 7 days of in vitro culture or 15 days of xenotransplantion. ALA was used at two different concentrations (100 μM: ALA100 and 150 μM: ALA150). Ovarian tissue was evaluated by classical histology (follicular morphology, development, and stromal cell density); immunohistochemistry for forkhead box O3a (FOXO3a); Ki67 (cell proliferation); cluster of differentiation 31 (CD31); and alpha smooth muscle actin (α-SMA). Reactive oxygen species (ROS) levels in ovarian tissue, as well as malondialdehyde (MDA) and nitrite levels in the culture medium, were assessed. Similar percentage of morphologically normal follicles was found in the vitrified ovarian tissue in the presence of ALA100 or ALA150 after in vitro culture or xenotransplantation. Follicular development from all treatments was higher (P < 0.05) than the control group. Moreover, an activation of primordial follicles was observed by FOXO3a. Stromal cell density and immunostaining for Ki67 and CD31 were significantly higher (P < 0.05) in ALA150 vitrified tissue. No difference (P > 0.05) was found in α-SMA between ALA concentrations after in vitro culture or xenograft. ROS levels in the ovarian tissue were similar (P > 0.05) in all treatments, as well as MDA and nitrite levels after 7 days of culture. We concluded that the addition of ALA 150 is able to better preserve the stromal cell density favoring granulosa cell proliferation and neovascularization.
(© 2021. Society for Reproductive Investigation.)
Databáze: MEDLINE
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