Two-Photon Microperimetry: A Media Opacity-Independent Retinal Function Assay.

Autor:	Wei A; UCI Health Gavin Herbert Eye Institute, Department of Ophthalmology, University of California-Irvine, Irvine, CA, USA., Mehta UV; UCI Health Gavin Herbert Eye Institute, Department of Ophthalmology, University of California-Irvine, Irvine, CA, USA.; College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA, USA., Palczewska G; UCI Health Gavin Herbert Eye Institute, Department of Ophthalmology, University of California-Irvine, Irvine, CA, USA.; Department of Medical Devices, Polgenix, Inc., Cleveland, OH, USA., Palma AM; Institute for Clinical and Translational Sciences, University of California-Irvine, Irvine, CA, USA., Hussey VM; UCI School of Medicine, University of California-Irvine, Irvine, CA, USA., Hoffmann LE; UCI School of Medicine, University of California-Irvine, Irvine, CA, USA., Diep A; UCI School of Medicine, University of California-Irvine, Irvine, CA, USA., Nguyen K; Creighton University School of Medicine, Omaha, NE, USA., Le B; Drexel University College of Medicine, Philadelphia, PA, USA., Chang SY; UCI School of Medicine, University of California-Irvine, Irvine, CA, USA., Browne AW; UCI Health Gavin Herbert Eye Institute, Department of Ophthalmology, University of California-Irvine, Irvine, CA, USA.; Institute for Clinical and Translational Sciences, University of California-Irvine, Irvine, CA, USA.; Department of Biomedical Engineering, University of California-Irvine, Irvine, CA, USA.
Jazyk:	angličtina
Zdroj:	Translational vision science & technology [Transl Vis Sci Technol] 2021 Feb 05; Vol. 10 (2), pp. 11.
DOI:	10.1167/tvst.10.2.11
Abstrakt:	Purpose: Compare results obtained using infrared two-photon microperimetry (2PM-IR) with conventional visual function tests in healthy subjects of varying ages with and without simulated media opacities. Methods: Subjects from two separate cohort studies completed cone contrast threshold (CCT) testing, conventional microperimetry, visible light microperimetry from a novel device (2PM-Vis), and infrared two-photon microperimetry. The first cohort study, which consisted of six healthy volunteers (23 to 29 years of age), evaluated the effects of simulated media opacities on visual performance testing. Subjects underwent testing on four visual function devices nine separate times under the following conditions: no filter, red filter, green filter, blue filter, light brown filter, dark brown filter, polarized black filter (0° rotation), and polarized black filter (90° rotation). Subjects subsequently performed 2PM-IR and 2PM-Vis testing without a filter in the mydriatic state. The second cohort study evaluated the effect of age on visual test performance in 42 healthy subjects split between two groups (ages 20-40 years and 60-80 years). Results: Retinal sensitivity measured by 2PM-IR demonstrated lower variability than all other devices relying on visible spectrum stimuli. Retinal sensitivity decreased proportionally with the transmittance of light through each filter. CCT scores and retinal sensitivity decreased with age in all testing modalities. Visible spectrum testing modalities demonstrated larger test result differences between young and old patient cohorts; this difference was inversely proportional to the wavelength of the visual function test. Conclusions: 2PM-IR mitigates media opacities that may mask small differences in retinal sensitivity when tested with conventional visual function testing devices. Translational Relevance: Conventional visual function tests that emit visible light may not detect differences in retinal function during the early stages of age-related diseases due to the confounding effects of cataracts. Infrared light, which has greater transmittance through ocular tissue, may reliably quantify retinal sensitivity and thereby detect degenerative changes early on.
Databáze:	MEDLINE
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