R7 photoreceptor axon targeting depends on the relative levels of lost and found expression in R7 and its synaptic partners.

Autor:	Douthit J; Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, NYU School of Medicine, New York, United States., Hairston A; Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, NYU School of Medicine, New York, United States., Lee G; Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, NYU School of Medicine, New York, United States., Morrison CA; Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, NYU School of Medicine, New York, United States., Holguera I; Department of Biology, New York University, New York, United States., Treisman JE; Kimmel Center for Biology and Medicine at the Skirball Institute and Department of Cell Biology, NYU School of Medicine, New York, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 May 18; Vol. 10. Date of Electronic Publication: 2021 May 18.
DOI:	10.7554/eLife.65895
Abstrakt:	As neural circuits form, growing processes select the correct synaptic partners through interactions between cell surface proteins. The presence of such proteins on two neuronal processes may lead to either adhesion or repulsion; however, the consequences of mismatched expression have rarely been explored. Here, we show that the Drosophila CUB-LDL protein Lost and found (Loaf) is required in the UV-sensitive R7 photoreceptor for normal axon targeting only when Loaf is also present in its synaptic partners. Although targeting occurs normally in loaf mutant animals, removing loaf from photoreceptors or expressing it in their postsynaptic neurons Tm5a/b or Dm9 in a loaf mutant causes mistargeting of R7 axons. Loaf localizes primarily to intracellular vesicles including endosomes. We propose that Loaf regulates the trafficking or function of one or more cell surface proteins, and an excess of these proteins on the synaptic partners of R7 prevents the formation of stable connections. Competing Interests: JD, AH, GL, CM, IH, JT No competing interests declared (© 2021, Douthit et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu