Low-level laser therapy (LLLT) improves alveolar bone healing in rats.

Autor: Ribeiro LNS; Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Av. Do Café S/N, Ribeirao Preto, SP, CEP: 14040-904, Brazil., de Figueiredo FAT; Department of Dental Materials and Prosthodontics, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Av. Do Café S/N, Ribeirao Preto, SP, CEP, 14040-904, Brazil. fellipe.figueiredo@usp.br., da Silva Mira PC; Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Av. Do Café S/N, Ribeirao Preto, SP, CEP: 14040-904, Brazil., Arnez MFM; Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Av. Do Café S/N, Ribeirao Preto, SP, CEP: 14040-904, Brazil., Matsumoto MAN; Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Av. Do Café S/N, Ribeirao Preto, SP, CEP: 14040-904, Brazil., de Menezes LM; Dental Program - School of Health and Life Sciences, Pontifical Catholic University of Rio Grande Do Sul, PUCRS, Porto Alegre, RS, Brazil., Küchler EC; Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Av. Do Café S/N, Ribeirao Preto, SP, CEP: 14040-904, Brazil., Stuani MBS; Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo (USP), Av. Do Café S/N, Ribeirao Preto, SP, CEP: 14040-904, Brazil.
Jazyk: angličtina
Zdroj: Lasers in medical science [Lasers Med Sci] 2022 Mar; Vol. 37 (2), pp. 961-969. Date of Electronic Publication: 2021 May 17.
DOI: 10.1007/s10103-021-03340-y
Abstrakt: The main objective of the present study was to evaluate the effect of low-level laser therapy (LLLT) in enhancing bone healing in irradiated alveolus post-tooth extraction. Sixty male Wistar rats (180 ± 10 g) were used in the present study. The left maxillary first molars were extracted, and the alveolar region was irradiated by diode laser device (GaAlAs) immediately after extraction and for more 3-day daily applications. The animals were randomly assigned into two groups: control group (n = 30, with left maxillary molar extraction-CG) and experimental group (n = 30, with tooth extraction and low-level laser therapy applied to the dental alveolus for 42 s-EG). These groups were divided into subgroups (five rats per subgroup) according to the observation time point-1, 2, 3, 5, 7, and 10 days-post-tooth extraction. The maxillary bone was separated, and the specimens were stained with hematoxylin and eosin, Masson's trichrome, and picrosirius red and immunohistochemistry for RUNX-2. Parametric and nonparametric tests were used with a significance level of 5%. LLLT accelerated bone healing with mature collagen fiber bundles and early new bone formation. Histomorphometric analysis revealed an increase of osteoblast (RUNX-2) and osteoclast (TRAP) activity and in the area percentage of cancellous bone in the lased alveolus compared to the control group. This increase was statistically significant (p < 0.05). Application of LLLT with a GaAlAs diode laser device enhanced bone healing and mineralization on alveolar region.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.)
Databáze: MEDLINE
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