Rapid isolation and immune profiling of SARS-CoV-2 specific memory B cell in convalescent COVID-19 patients via LIBRA-seq.

Autor:	He B; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Liu S; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Wang Y; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Xu M; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Cai W; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Liu J; Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China., Bai W; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Ye S; Pulmonary and critical care medicine, The Third People's Hospital of Dongguan City, Dongguan, Guangdong Province, China., Ma Y; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Hu H; Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China., Meng H; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Sun T; Hangzhou ImmuQuad Biotechnologies, Hangzhou, China.; Zhejiang-California International NanoSystems Institute, Zhejiang University, Hangzhou, China., Li Y; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Luo H; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Shi M; Infection and Immunity Center, School of Medicine, Sun Yat-sen University, Shenzhen, China., Du X; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China., Zhao W; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, School of Medicine, Sun Yat-sen University, Guangzhou, China., Chen S; Guangdong Provincial Key Laboratory of Biomedical Imaging, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, China., Yang J; Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China., Zhu H; Department of Infectious Diseases, The Ninth People's Hospital of Dongguan City, Dongguan, Guangdong Province, China., Jie Y; Department of Infectious Diseases, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China., Yang Y; School of Data and Computer Science, Sun Yat-sen University, Guangzhou, China., Guo D; Infection and Immunity Center, School of Medicine, Sun Yat-sen University, Shenzhen, China., Wang Q; Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China., Liu Y; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Shenzhen, China.; Genome Analysis Laboratory of the Ministry of Agriculture, Shenzhen, China.; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China., Yan H; Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China., Wang M; Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China., Chen YQ; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, China. chenyaoqing@mail.sysu.edu.cn.; Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, China. chenyaoqing@mail.sysu.edu.cn.
Jazyk:	angličtina
Zdroj:	Signal transduction and targeted therapy [Signal Transduct Target Ther] 2021 May 17; Vol. 6 (1), pp. 195. Date of Electronic Publication: 2021 May 17.
DOI:	10.1038/s41392-021-00610-7
Abstrakt:	B cell response plays a critical role against SARS-CoV-2 infection. However, little is known about the diversity and frequency of the paired SARS-CoV-2 antigen-specific BCR repertoire after SARS-CoV-2 infection. Here, we performed single-cell RNA sequencing and VDJ sequencing using the memory and plasma B cells isolated from five convalescent COVID-19 patients, and analyzed the spectrum and transcriptional heterogeneity of antibody immune responses. Via linking BCR to antigen specificity through sequencing (LIBRA-seq), we identified a distinct activated memory B cell subgroup (CD11c high CD95 high ) had a higher proportion of SARS-CoV-2 antigen-labeled cells compared with memory B cells. Our results revealed the diversity of paired BCR repertoire and the non-stochastic pairing of SARS-CoV-2 antigen-specific immunoglobulin heavy and light chains after SARS-CoV-2 infection. The public antibody clonotypes were shared by distinct convalescent individuals. Moreover, several antibodies isolated by LIBRA-seq showed high binding affinity against SARS-CoV-2 receptor-binding domain (RBD) or nucleoprotein (NP) via ELISA assay. Two RBD-reactive antibodies C14646P3S and C2767P3S isolated by LIBRA-seq exhibited high neutralizing activities against both pseudotyped and authentic SARS-CoV-2 viruses in vitro. Our study provides fundamental insights into B cell response following SARS-CoV-2 infection at the single-cell level.
Databáze:	MEDLINE
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