Hyaluronic Acid Concentration in Female Vocal Folds With Reinke's Edema.

Autor: Lins CVM; Department of Otorhinolaryngology-Head and Neck Surgery, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil., Maciel Martins JR; Laboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil., Kobayashi EY; Laboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil., Korn GP; Department of Otorhinolaryngology-Head and Neck Surgery, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil., Park SW; Department of Otorhinolaryngology-Head and Neck Surgery, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil., Mororó WC; Department of Otorhinolaryngology-Head and Neck Surgery, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil.; Hospital Universitário da Universidade Federal do Maranhão, São Luís, Brazil., De Biase NG; Department of Otorhinolaryngology-Head and Neck Surgery, Universidade Federal de São Paulo / Escola Paulista de Medicina, São Paulo, Brazil.; Pontifícia Universidade Católica de São Paulo, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery [Otolaryngol Head Neck Surg] 2022 Feb; Vol. 166 (2), pp. 337-342. Date of Electronic Publication: 2021 May 18.
DOI: 10.1177/01945998211008914
Abstrakt: Objective: The aim of the present study was to investigate hyaluronic acid (HA) concentrations in vocal folds among patients with Reinke's edema.
Study Design: Prospective and experimental study.
Setting: Single tertiary center.
Methods: An HA binding protein isolated from bovine nasal cartilage was used to identify and isolate the HA from samples. Plates coated with biotin-conjugated binding protein and streptavidin-europium conjugate were sequentially incubated with 18 Reinke's edema samples and 11 female vocal fold cover samples from cadavers (the superficial layer of the lamina propria; control group). After the release of europium from streptavidin in enhancement solution, final fluorescence was measured in a fluorometer.
Results: The mean HA concentration in Reinke's edema vocal folds was significantly higher than that in the control vocal folds (9.2 × 10 3 vs 0.9 × 10 3 µg/g).
Conclusion: Vocal fold covers affected by Reinke's edema present a higher concentration of HA than do vocal fold covers with no edema.
Databáze: MEDLINE
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