On-line solid phase extraction-ultra-high performance liquid chromatography coupled to tandem mass spectrometry for the determination of N-nitrosodiethanolamine in baby shampoo.

Autor: Tada A; Brazilian Federal Institute, Brasília, DF, Brazil., Rodrigues-Silva C; Institute of Chemistry, Department of Analytical Chemistry, University of Campinas, P.O. Box 6154, 13084-971, Campinas, SP, Brazil., Rath S; Institute of Chemistry, Department of Analytical Chemistry, University of Campinas, P.O. Box 6154, 13084-971, Campinas, SP, Brazil. Electronic address: rath@unicamp.br.
Jazyk: angličtina
Zdroj: Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2021 Aug 05; Vol. 202, pp. 114132. Date of Electronic Publication: 2021 May 11.
DOI: 10.1016/j.jpba.2021.114132
Abstrakt: N-nitrosodiethanolamine (NDELA) is a carcinogenic contaminant of concern in the cosmetics industry. Contaminated raw material, degradation, reactions of ingredients of the formulation, or migration of packaging material can be responsible for the presence of NDELA in the final product. Liquid chromatography coupled to tandem mass spectrometry is the most widely accepted technique for the quantitation of NDELA in cosmetic products. Still, there is no consensus regarding the sample preparation procedure. The aim of this work was to evaluate the performance of two-dimensional liquid chromatography coupled with tandem mass spectrometry for the determination of NDELA in shampoo. In the first dimension an Oasis HLB SPE-column was used and in the second dimension a CSH C18 column. NDELA-d 8 was used as an internal standard. The 2D-LC parameters were optimized by a central composite multivariate design. However, before quantitation, a sample preparation step using solid-phase extraction was necessary to eliminate compounds present in the formulation, especially surfactants that were not compatible with the chromatographic columns. Moreover, the complex matrices and singular compositions of shampoo from different manufacturers required adjustments of the sample preparation procedure for each sample. The limit of quantitation of the method for the determination of NDELA in shampoo was in the range of 5-10 ng g -1 . The accuracy of the method at the LOQ (10 ng g -1 ) was 114 % and the inter-day precision of 15.3 % (n = 9). One sample out of 12 presented an NDELA concentration of 54 ng g -1 .
Competing Interests: Declaration of Competing Interest The authors report no declarations of interest.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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