Expression of Myoglobin in Normal and Cancer Brain Tissues: Correlation With Hypoxia Markers.

Autor:	Elsherbiny ME; Department of Pharmacology and Toxicology, Ahram Canadian University, 6th of October, Egypt., Shaaban M; Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt., El-Tohamy R; Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt., Elkholi IE; Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt., Hammam OA; Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt., Magdy M; Department of Pathology, Theodor Bilharz Research Institute, Giza, Egypt., Allalunis-Turner J; Department of Oncology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB, Canada., Emara M; Center for Aging and Associated Diseases, Zewail City of Science, Technology and Innovation, 6th of October, Egypt.
Jazyk:	angličtina
Zdroj:	Frontiers in oncology [Front Oncol] 2021 Apr 30; Vol. 11, pp. 590771. Date of Electronic Publication: 2021 Apr 30 (Print Publication: 2021).
DOI:	10.3389/fonc.2021.590771
Abstrakt:	Background: Myoglobin (MB) is increasingly recognized as a key player in cancer growth and metastasis. Low oxygen tensions, commonly associated with highly aggressive and recurrent cancers, have been shown to regulate its expression in several cancers such as lung, neck, prostate and breast cancer. However, it is not yet known whether it contributes to the growth and spread of brain cancers especially Glioblastoma multiforme (GBM). Methods: Here we investigate the expression of MB, and its correlation with the hypoxia markers carbonic anhydrase IX (CAIX) and lactate dehydrogenase A (LDHA), in human tissue microarrays of multiple organ tumors, brain tumors, and GBM tumors, and their respective cancer-adjacent normal tissues. Correlation between MB protein expression and tumor grade was also assessed. Results: We show that MB protein is expressed in a wide variety of cancers, benign tumors, cancer-adjacent normal tissues, hyperplastic tissue samples and normal brain tissue, and low oxygen tensions modulate MB protein expression in different brain cancers, including GBM. Enhanced nuclear LDHA immune-reactivity in GBM was also observed. Finally, we report for the first time a positive correlation between MB expression and brain tumor grade. Conclusion: Our data suggest that hypoxia regulate MB expression in different brain cancers (including GBM) and that its expression is associated with a more aggressive phenotype as indicated by the positive correlation with the brain tumor grade. Additionally, a role for nuclear LDHA in promoting aggressive tumor phenotype is also suggested based on enhanced nuclear expression which was observed only in GBM. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Elsherbiny, Shaaban, El-Tohamy, Elkholi, Hammam, Magdy, Allalunis-Turner and Emara.)
Databáze:	MEDLINE
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