Clinical integration of fast Raman spectroscopy for Mohs micrographic surgery of basal cell carcinoma.

Autor:	Boitor R; School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom., de Wolf C; Department of Dermatology, Erasmus MC, Rotterdam 3015 GD, The Netherlands., Weesie F; Department of Dermatology, Erasmus MC, Rotterdam 3015 GD, The Netherlands., Shipp DW; School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom., Varma S; Nottingham NHS Treatment Centre, Nottingham University Hospitals, Lister Rd, Nottingham NG7 2FT, United Kingdom., Veitch D; Nottingham NHS Treatment Centre, Nottingham University Hospitals, Lister Rd, Nottingham NG7 2FT, United Kingdom., Wernham A; Nottingham NHS Treatment Centre, Nottingham University Hospitals, Lister Rd, Nottingham NG7 2FT, United Kingdom., Koloydenko A; Mathematics Department, Royal Holloway University of London, Egham, TW20 OEX, United Kingdom., Puppels G; Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus MC, Rotterdam 3015 GD, The Netherlands.; RiverD International B.V., Marconistraat 16, Rotterdam 3029 AK, The Netherlands., Nijsten T; Department of Dermatology, Erasmus MC, Rotterdam 3015 GD, The Netherlands., Williams HC; Centre for Evidence Based Dermatology, Nottingham University Hospital NHS Trust, QMC Campus, Derby Road, Nottingham NG7 2UH, United Kingdom., Caspers P; Center for Optical Diagnostics and Therapy, Department of Dermatology, Erasmus MC, Rotterdam 3015 GD, The Netherlands.; RiverD International B.V., Marconistraat 16, Rotterdam 3029 AK, The Netherlands., Notingher I; School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2021 Mar 11; Vol. 12 (4), pp. 2015-2026. Date of Electronic Publication: 2021 Mar 11 (Print Publication: 2021).
DOI:	10.1364/BOE.417896
Abstrakt:	We present the first clinical integration of a prototype device based on integrated auto-fluorescence imaging and Raman spectroscopy (Fast Raman device) for intra-operative assessment of surgical margins during Mohs micrographic surgery of basal cell carcinoma (BCC). Fresh skin specimens from 112 patients were used to optimise the tissue pre-processing and the Fast Raman algorithms to enable an analysis of complete Mohs layers within 30 minutes. The optimisation allowed >95% of the resection surface area to be investigated (including the deep and epidermal margins). The Fast Raman device was then used to analyse skin layers excised from the most relevant anatomical sites (nose, temple, eyelid, cheek, forehead, eyebrow and lip) and to detect the three main types of BCC (nodular, superficial and infiltrative). These results suggest that the Fast Raman technique is a promising tool to provide an objective diagnosis "tumour clear yes/no" during Mohs surgery of BCC. This clinical integration study is a key step towards a larger scale diagnosis test accuracy study to reliably determine the sensitivity and specificity in a clinical setting. Competing Interests: I Notingher, H Williams, S Varma, A. Koloydenko hold a patent related to the Fast Raman technology. The Fast Raman device was built by RiverD International. (Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.)
Databáze:	MEDLINE
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