Streptococcus pneumoniae prevalence in nasopharynx, oropharynx and gingival sulcus in Brazilian adults:A preliminary study.

Autor: Lomardo PG; Post-graduation in Dentistry, School of Dentistry, Universidade Federal Fluminense, Brazil., Cardoso-Marques NT; Department of Microbiology and Parasitology, Biomedical Institute, Universidade Federal Fluminense, Brazil., Motta LA; Post-graduation in Dentistry, School of Dentistry, Universidade Federal Fluminense, Brazil., Aguiar TRS; Post-graduation in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Brazil., Neves FPG; Department of Microbiology and Parasitology, Biomedical Institute, Universidade Federal Fluminense, Brazil., Aguiar-Alves F; Post-graduation Program in Applied Microbiology and Parasitology, Universidade Federal Fluminense, Brazil., Calasans-Maia MD; Post-graduation in Dentistry, School of Dentistry, Universidade Federal Fluminense, Brazil., Quinelato V; Post-graduation in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Brazil., Casado PL; Post-graduation in Dentistry, School of Dentistry, Universidade Federal Fluminense, Brazil; Post-graduation in Implant Dentistry, School of Dentistry, Universidade Federal Fluminense, Brazil. Electronic address: priscilacasado@id.uff.br.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2021 Jul; Vol. 156, pp. 104924. Date of Electronic Publication: 2021 May 13.
DOI: 10.1016/j.micpath.2021.104924
Abstrakt: Aim: This study aimed to evaluate the prevalence of S. pneumoniae colonization in three different sites in healthy adults: nasopharynx, oropharynx and gingival sulcus.
Methods: Two-hundred and sixty five adults, aged 20-60 years, who attended dental clinics in one public university (n = 106) and one military institution (n = 159) were enrolled in this study. Pneumococcal detection was performed by direct culture (DC) and PCR for lytA gene after a broth enrichment step. Capsular types were determined by sequential multiplex PCR.
Results: We identified 18 (6.8%) pneumococcal carriers among 265 adults by PCR, but only one (0.4%) pneumococcal strain was isolated by DC method. Oropharynx (17; 6.4%) was the main source of S. pneumoniae. Colonization of gingival sulcus and nasopharynx was found in 4 (1.5%) and 2 (0.8%) adults, respectively. Nine distinct capsular types were detected from 9 adults and co-colonization with 2 serotypes was confirmed in 4 (1.5%) subjects. Factors associated with carriage were being females, low level of schooling, non-military and regular medication. We observed a low (6.8%) pneumococcal carriage prevalence, but oropharyngeal samples yielded more sensitive results, especially by the PCR-based detection methodology.
Conclusion: Gingival sulcus was found to be a possible reservoir for S. pneumoniae independently of the oropharynx or nasopharynx colonization.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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