Morphological and molecular differentiation of Diplostomum spp. metacercariae from brain of minnows (Phoxinus phoxinus L.) in four populations of northern Europe and East Asia.

Autor: Lebedeva DI; Institute of Biology, Karelian Research Center, Russian Academy of Sciences, Pushkinskaya St. 11, 185910 Petrozavodsk, Russia. Electronic address: daryal78@gmail.com., Chrisanfova GG; Institute of Gene Biology, Russian Academy of Sciences, Vavilov str., 34/5, 119334 Moscow, Russia., Ieshko EP; Institute of Biology, Karelian Research Center, Russian Academy of Sciences, Pushkinskaya St. 11, 185910 Petrozavodsk, Russia., Guliaev AS; Institute of Gene Biology, Russian Academy of Sciences, Vavilov str., 34/5, 119334 Moscow, Russia., Yakovleva GA; Institute of Biology, Karelian Research Center, Russian Academy of Sciences, Pushkinskaya St. 11, 185910 Petrozavodsk, Russia., Mendsaikhan B; Institute of Geography and Geoecology, Mongolian Academy of Sciences,РО Box 361, 214192 Ulaanbaatar, Mongolia., Semyenova SK; Institute of Gene Biology, Russian Academy of Sciences, Vavilov str., 34/5, 119334 Moscow, Russia.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2021 Aug; Vol. 92, pp. 104911. Date of Electronic Publication: 2021 May 12.
DOI: 10.1016/j.meegid.2021.104911
Abstrakt: Metacercariae of trematodes from the genus Diplostomum are major helminth pathogens of freshwater fish, infecting the eye or the brain. The taxonomy of the genus Diplostomum is complicated, and has recently been based mainly on the molecular markers. In this study, we report the results of the morphological and molecular genetic analysis of diplostomid metacercaria from the brain of the minnow Phoxinus phoxinus from three populations in Fennoscandia (Northern Europe) and one population in Mongolia (East Asia). We obtained the data on the polymorphism of the partial mitochondrial cox1 gene and ribosomal ITS1-5.8S-ITS2 region of these parasites. РСА-based morphological analysis revealed that the parasites in the Asian and the European groups of Diplostomum sp. were distinctly different. Metacercariae from the brain of Mongolian minnows were much larger than those from the brain of Fennoscandian minnows but had much fewer excretory granules. Considering that the two study regions were separated by a distance of about 4500 km, we also tested the genetic homogeneity of their host, the minnow, using the mitochondrial cytb gene. It was shown that Diplostomum-infected minnows from Mongolia and Fennoscandia represented two previously unknown separate phylogenetic lineages of the genus Phoxinus. Both molecular and morphological analysis demonstrated that the parasites from Fennoscandia belonged the species Diplostomum phoxini, while the parasites from Mongolia belonged to a separate species, Diplostomum sp. MТ.Each of the two studied Diplostomum spp. was associated with a specific, and previously unknown, genealogical lineage of its second intermediate host, P. phoxinus.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: