Spatiotemporal control of cell cycle acceleration during axolotl spinal cord regeneration.

Autor:	Cura Costa E; Systems Biology Group (SysBio), Institute of Physics of Liquids and Biological Systems (IFLySIB), National Scientific and Technical Research Council (CONICET) and University of La Plata (UNLP), La Plata, Argentina., Otsuki L; The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria., Rodrigo Albors A; Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee, Dundee, United Kingdom., Tanaka EM; The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria., Chara O; Systems Biology Group (SysBio), Institute of Physics of Liquids and Biological Systems (IFLySIB), National Scientific and Technical Research Council (CONICET) and University of La Plata (UNLP), La Plata, Argentina.; Center for Information Services and High Performance Computing, Technische Universität Dresden, Dresden, Germany.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 May 14; Vol. 10. Date of Electronic Publication: 2021 May 14.
DOI:	10.7554/eLife.55665
Abstrakt:	Axolotls are uniquely able to resolve spinal cord injuries, but little is known about the mechanisms underlying spinal cord regeneration. We previously found that tail amputation leads to reactivation of a developmental-like program in spinal cord ependymal cells (Rodrigo Albors et al., 2015), characterized by a high-proliferation zone emerging 4 days post-amputation (Rost et al., 2016). What underlies this spatiotemporal pattern of cell proliferation, however, remained unknown. Here, we use modeling, tightly linked to experimental data, to demonstrate that this regenerative response is consistent with a signal that recruits ependymal cells during ~85 hours after amputation within ~830 μm of the injury. We adapted Fluorescent Ubiquitination-based Cell Cycle Indicator (FUCCI) technology to axolotls (AxFUCCI) to visualize cell cycles in vivo. AxFUCCI axolotls confirmed the predicted appearance time and size of the injury-induced recruitment zone and revealed cell cycle synchrony between ependymal cells. Our modeling and imaging move us closer to understanding bona fide spinal cord regeneration. Competing Interests: EC, LO, AR, ET, OC No competing interests declared (© 2021, Cura Costa et al.)
Databáze:	MEDLINE
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