Potential of watermelon ( Citrullus lanatus ) to maintain oxidative stability of rooster semen for artificial insemination.

Autor:	Jimoh OA; Department of Agricultural Technology, The Federal Polytechnic Ado-Ekiti, Ekiti State 360101, Nigeria., Akinola MO; Department of Agricultural Technology, The Federal Polytechnic Ado-Ekiti, Ekiti State 360101, Nigeria., Oyeyemi BF; Molecular Biology Group, Department of Science Technology, The Federal Polytechnic Ado-Ekiti, Ekiti State 360101, Nigeria., Oyeyemi WA; Department of Physiology, Igbenedion University, Okada, Edo State 302110, Nigeria., Ayodele SO; Department of Agricultural Technology, The Federal Polytechnic Ado-Ekiti, Ekiti State 360101, Nigeria., Omoniyi IS; Department of Agricultural Technology, The Federal Polytechnic Ado-Ekiti, Ekiti State 360101, Nigeria., Okin-Aminu HO; Animal Science Department, Ahmadu Bello University, Zaria, Kaduna State 810241, Nigeria.
Jazyk:	angličtina
Zdroj:	Journal of animal science and technology [J Anim Sci Technol] 2021 Jan; Vol. 63 (1), pp. 46-57. Date of Electronic Publication: 2021 Jan 31.
DOI:	10.5187/jast.2021.e21
Abstrakt:	Fruits with antioxidant enrichment can be an economically affordable supplement for mitigating oxidative damage prone spermatozoa membrane pathologies. Computer-assisted sperm analyzer and oxidative status were utilized to evaluate the impact of watermelon ( Citrullus lanatus ) fortification of dextrose saline as diluent for rooster semen and fertility response of hens inseminated. Watermelon juice and dextrose saline were used to formulate diluent of 7 treatments consisting of unextended semen (positive control), 10%, 20%, 30%, 40%, 50% and only dextrose saline (negative control) designated as Treatments 1-7. Pooled semen was obtained from fertile roosters and equilibrated with diluents at ratio 1:2 in the various treatments and were evaluated using computer software coupled microscope and seminal oxidative status assay. 168 laying hens randomly divided into 7 treatment of 8 replicates and 3 hen per replicate. Hen were everted, and semen (2 × 10 8 Spermatozoa) deposited intra-vagina and eggs collected over 8 weeks to assess fertility and hatchability of eggs laid. The result obtained revealed that watermelon-dextrose saline rooster semen diluent enhanced progressive motility, sperm kinetics and lowered non-progressive motility in T2-T6 compared to T7 over the 3 hours of evaluation. Watermelon addition to rooster semen diluent enhance the antioxidant capacity of rooster semen and lowered lipid peroxide generation. The percentage fertility was highest in T3 (81.01%) and T4 (81.24%) with lowest value obtained in T7 (73.46%). The hatchability of eggs set of hens inseminated with undiluted semen (71.46%) was lower than values for hens inseminated with watermelon inclusive extended semen (75.71%-80.39%). The optimal inclusion of 30%-40% watermelon in dextrose saline diluent enhance rooster semen kinetics, seminal oxidative stability and egg fertility. Competing Interests: No potential conflict of interest relevant to this article was reported. (© Copyright 2021 Korean Society of Animal Science and Technology.)
Databáze:	MEDLINE
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