The regenerative effect of different growth factors and platelet lysate on meniscus cells and mesenchymal stromal cells and proof of concept with a functionalized meniscus implant.

Autor:	Hagmeijer MH; Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands., Korpershoek JV; Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands., Crispim JF; Developmental Bioengineering, University of Twente, Enschede, The Netherlands.; Department of Molecules and Materials, MESA+ Institute for Nanotechnology, University of Twente, Enschede, The Netherlands., Chen LT; Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands., Jonkheijm P; Department of Molecules and Materials, MESA+ Institute for Nanotechnology, University of Twente, Enschede, The Netherlands., Krych AJ; Department of Orthopedic Surgery and Sports Medicine, Mayo Clinic, Rochester, Minnesota, USA., Saris DBF; Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands.; Developmental Bioengineering, University of Twente, Enschede, The Netherlands.; Department of Orthopedic Surgery and Sports Medicine, Mayo Clinic, Rochester, Minnesota, USA., Vonk LA; Department of Orthopedics, University Medical Center Utrecht, Utrecht, The Netherlands.
Jazyk:	angličtina
Zdroj:	Journal of tissue engineering and regenerative medicine [J Tissue Eng Regen Med] 2021 Jul; Vol. 15 (7), pp. 648-659. Date of Electronic Publication: 2021 May 21.
DOI:	10.1002/term.3218
Abstrakt:	Meniscus regeneration could be enhanced by targeting meniscus cells and mesenchymal stromal cells (MSCs) with the right growth factors. Combining these growth factors with the Collagen Meniscus Implant (CMI®) could accelerate cell ingrowth and tissue formation in the implant and thereby improve clinical outcomes. Using a transwell migration assay and a micro-wound assay, the effect of insulin-like growth factor-1, platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), transforming growth factor beta 1 (TGF-β1), fibroblast growth factor, and platelet lysate (PL) on migration and proliferation of meniscus cells and MSCs was assessed. The formation of extracellular matrix under influence of the above-mentioned growth factors was assessed after 28 days of culture of both MSCs and meniscus cells. As a proof of concept, the CMI® was functionalized with a VEGF binding peptide and coated with platelet-rich plasma (PRP) for clinical application. Our results demonstrate that PDGF, TGF-β1, and PL stimulate migration, proliferation, and/or extracellular matrix production of meniscus cells and MSCs. Additionally, the CMI® was successfully functionalized with a VEGF binding peptide and PRP which increased migration of meniscus cell and MSC into the implant. This study demonstrates proof of concept of functionalizing the CMI® with growth factor binding peptides. A CMI® functionalized with the right growth factors holds great potential for meniscus replacement after partial meniscectomy. (© 2021 The Authors. Journal of Tissue Engineering and Regenerative Medicine published by John Wiley & Sons Ltd.)
Databáze:	MEDLINE
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