Biochemical markers for pregnancy in the spent culture medium of in vitro produced bovine embryos†.

Autor:	de Oliveira Fernandes G; School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil., Milazzotto MP; Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, São Paulo, Brazil., Fidelis AAG; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil., Kawamoto TS; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil., de Oliveira Leme L; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil.; UFES, Universidade Federal do Espírito Santo, Alegre, ES, Brazil., de Lima CB; Center of Natural and Human Sciences, Universidade Federal do ABC, Santo André, São Paulo, Brazil.; Centre de Recherche en Reproduction, Développement et Santé Intergénérationnelle. Département des Sciences Animales, Université Laval, Québec, Canada., Franco MM; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil., Dode MAN; School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.; Laboratory of Animal Reproduction, Embrapa Genetic Resources and Biotechnology, Brasília, Brazil.
Jazyk:	angličtina
Zdroj:	Biology of reproduction [Biol Reprod] 2021 Aug 03; Vol. 105 (2), pp. 481-490.
DOI:	10.1093/biolre/ioab095
Abstrakt:	The present study aimed to identify biomarkers to assess the quality of in vitro produced (IVP) bovine embryos in the culture media. IVP embryos on Day (D) 5 of development were transferred to individual drops, where they were maintained for the last 48 h of culture. Thereafter, the medium was collected and the embryos were transferred to the recipients. After pregnancy diagnosis, the media were grouped into the pregnant and nonpregnant groups. The metabolic profiles of the media were analyzed via electrospray ionization mass spectrometry, and the concentrations of pyruvate, lactate, and glutamate were assessed using fluorimetry. The spectrometric profile revealed that the media from embryos from the pregnant group presented a higher signal intensity compared to that of the nonpregnant group; the ions 156.13 Da [M + H]+, 444.33 Da [M + H]+, and 305.97 Da [M + H]+ were identified as biomarkers. Spent culture medium from expanded blastocysts (Bx) that established pregnancy had a greater concentration of pyruvate (p = 0.0174) and lesser concentration of lactate (p = 0.042) than spent culture medium from Bx that did not establish pregnancy. Moreover, pyruvate in the culture media of Bx can predict pregnancy with 90.9% sensitivity and 75% specificity. In conclusion, we identified markers in the culture media that helped in assessing the most viable IVP embryos with a greater potential to establish pregnancy. (© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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