Blood neutrophils from children with COVID-19 exhibit both inflammatory and anti-inflammatory markers.

Autor:	Seery V; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA, Facultad de Medicina. UBA-CONICET, Paraguay 2155, C1121ABG CABA, Argentina., Raiden SC; Departamento de Medicina, Hospital General de Niños Pedro de Elizalde. Av. Montes de Oca 40, CABA C1270, Argentina., Algieri SC; Servicio de Pediatría, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Grisolía NA; Departamento de Medicina, Hospital General de Niños Pedro de Elizalde. Av. Montes de Oca 40, CABA C1270, Argentina., Filippo D; Servicio de Pediatría, Hospital Municipal Diego Thompson. Avellaneda 33, Buenos Aires B1650, Argentina., De Carli N; Servicio de Pediatría, Clínica del Niño de Quilmes, Av. Lamadrid 444, Buenos Aires B1878, Argentina., Di Lalla S; Departamento de Consultorios Externos, Hospital General de Niños Pedro de Elizalde, Av. Montes de Oca 40, CABA C1270, Argentina., Cairoli H; Departamento de Medicina, Hospital General de Niños Pedro de Elizalde. Av. Montes de Oca 40, CABA C1270, Argentina., Chiolo MJ; Departamento de Cirugía, Hospital General de Niños Pedro de Elizalde, Av. Montes de Oca 40, CABA C1270, Argentina., Meregalli CN; Unidad de Terapia Intensiva Pediátrica, Departamento de Urgencias, Hospital General de Niños Pedro de Elizalde. Av. Montes de Oca 40, CABA C1270, Argentina., Gimenez LI; Servicio de Pediatría, Hospital Municipal Diego Thompson. Avellaneda 33, Buenos Aires B1650, Argentina., Gregorio G; Servicio de Pediatría, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Sarli M; Unidad de Terapia Intensiva Pediátrica, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Alcalde AL; Servicio de Pediatría, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Davenport C; Departamento de Medicina, Hospital General de Niños Pedro de Elizalde. Av. Montes de Oca 40, CABA C1270, Argentina., Bruera MJ; Unidad de Terapia Intensiva Pediátrica, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Simaz N; Servicio de Pediatría, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Pérez MF; Servicio de Pediatría, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Nivela V; Departamento de Emergencias Pediátrica, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Bayle C; Departamento de Emergencias Pediátrica, Hospital Nacional Profesor Alejandro Posadas, Marconi Morón 386, Buenos Aires B1684, Argentina., Tuccillo P; Servicio de Pediatría, Hospital Naval Cirujano Mayor Dr. Pedro Mallo, Av. Patricias Argentinas 351, CABA C1405, Argentina., Agosta MT; Servicio de Pediatría, Hospital Naval Cirujano Mayor Dr. Pedro Mallo, Av. Patricias Argentinas 351, CABA C1405, Argentina., Pérez H; Servicio de Pediatría, Hospital Naval Cirujano Mayor Dr. Pedro Mallo, Av. Patricias Argentinas 351, CABA C1405, Argentina., Villa Nova S; Servicio de Pediatría, Hospital General de Agudos Dr. Juan A. Fernández, Av. Cerviño 3356, CABA C1425, Argentina., Suárez P; Servicio de Pediatría, Hospital General de Agudos Dr. Juan A. Fernández, Av. Cerviño 3356, CABA C1425, Argentina., Takata EM; Servicio de Pediatría, Hospital General de Agudos Dr. Juan A. Fernández, Av. Cerviño 3356, CABA C1425, Argentina., García M; Servicio de Pediatría, Hospital General de Agudos Dr. Juan A. Fernández, Av. Cerviño 3356, CABA C1425, Argentina., Lattner J; Servicio de Infectología Pediátrica, Hospital Naval Cirujano Mayor Dr. Pedro Mallo, Av. Patricias Argentinas 351, CABA C1405, Argentina., Rolón MJ; División Infectología, Hospital General de Agudos Dr. Juan A. Fernández, Av. Cerviño 3356, CABA C1425, Argentina., Coll P; División Infectología, Hospital General de Agudos Dr. Juan A. Fernández, Av. Cerviño 3356, CABA C1425, Argentina., Sananez I; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA, Facultad de Medicina. UBA-CONICET, Paraguay 2155, C1121ABG CABA, Argentina., Holgado MP; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA, Facultad de Medicina. UBA-CONICET, Paraguay 2155, C1121ABG CABA, Argentina., Ferrero F; Departamento de Medicina, Hospital General de Niños Pedro de Elizalde. Av. Montes de Oca 40, CABA C1270, Argentina., Geffner J; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA, Facultad de Medicina. UBA-CONICET, Paraguay 2155, C1121ABG CABA, Argentina., Arruvito L; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA, Facultad de Medicina. UBA-CONICET, Paraguay 2155, C1121ABG CABA, Argentina. Electronic address: arruvitol@gmail.com.
Jazyk:	angličtina
Zdroj:	EBioMedicine [EBioMedicine] 2021 May; Vol. 67, pp. 103357. Date of Electronic Publication: 2021 May 09.
DOI:	10.1016/j.ebiom.2021.103357
Abstrakt:	Background: Perhaps reflecting that children with COVID-19 rarely exhibit severe respiratory symptoms and often remain asymptomatic, little attention has been paid to explore the immune response in pediatric COVID-19. Here, we analyzed the phenotype and function of circulating neutrophils from children with COVID-19. Methods: An observational study including 182 children with COVID-19, 21 children with multisystem inflammatory syndrome (MIS-C), and 40 healthy children was performed in Buenos Aires, Argentina. Neutrophil phenotype was analyzed by flow cytometry in blood samples. Cytokine production, plasma levels of IgG antibodies directed to the spike protein of SARS-CoV-2 and citrullinated histone H3 were measured by ELISA. Cell-free DNA was quantified by fluorometry. Findings: Compared with healthy controls, neutrophils from children with COVID-19 showed a lower expression of CD11b, CD66b, and L-selectin but a higher expression of the activation markers HLA-DR, CD64 and PECAM-1 and the inhibitory receptors LAIR-1 and PD-L1. No differences in the production of cytokines and NETs were observed. Interestingly, the expression of CD64 in neutrophils and the serum concentration of IgG antibodies directed to the spike protein of SARS-CoV-2 distinguished asymptomatic from mild and moderate COVID-19. Interpretation: Acute lung injury is a prominent feature of severe COVID-19 in adults. A low expression of adhesion molecules together with a high expression of inhibitory receptors in neutrophils from children with COVID-19 might prevent tissue infiltration by neutrophils preserving lung function. Funding: This study was supported by the Ministry of Science and Technology (National Agency for Scientific and Technological Promotion, IP-COVID-19-0277 and PMO BID PICT 2018-2548), and University of Buenos Aires from Argentina (20020170100573BA). Competing Interests: Declaration of Competing Interest The authors have declared that no conflict of interest exists. (Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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