Determination of in vitro human whole blood-to-plasma ratio of THJ-018 utilizing gas chromatography-Mass spectrometry.

Autor: Kumar S; Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia., Khleel Alkhibery RN; Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia., Bakdash A; Department of Forensic Sciences, College of Criminal Justice, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia., Mohamed Abdelhady MS; Riyadh Poisons Control & Medical Forensic Chemistry Center, Kaab Ibn Al Harith, Ar Rawdah, Riyadh, Saudi Arabia; Egyptian Drug Authority, National Organizations for Drug Control and Research (NODCAR), Giza, Egypt.
Jazyk: angličtina
Zdroj: Indian journal of pharmacology [Indian J Pharmacol] 2021 Jan-Feb; Vol. 53 (1), pp. 13-18.
DOI: 10.4103/ijp.IJP_668_19
Abstrakt: Objectives: This study was aimed to determine in vitro human whole blood-to-plasma ratio (K WB / P ) of THJ-018 by gas chromatography/mass spectrometry (GC/MS).
Materials and Methods: The samples (human blood) were sprayed with THJ-018 and an internal standard and extracted using solid-phase extraction. THJ-018 was determined in the final extracts by GC/MS.
Results: The value for K WB / P was 1.56 (1.38-1.81), and red blood cell partitioning was 1.01 (1.01-1.02). The distribution of THJ-018 between whole blood and plasma was observed to be affected by temperature.
Conclusion: The data analysis supports the proposition that the ratio of the plasma to whole blood concentrations (1.56) is a suitable parameter characterizing THJ-018 distribution in whole blood. For toxicological analysis, it would be best to refrain from converting any drug concentration measured in whole blood to that anticipated in plasma or serum; however, toxic and therapeutic concentrations should be determined for the individual specimens collected.
Competing Interests: None
Databáze: MEDLINE
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