Quality Improvement of Post-Thawed Stallion Epididymal Spermatozoa with Single Layer Centrifugation.

Autor:	Cunha Dos Santos FC; Programa de Pos Graduacao Medicina Animal: Equinos, Faculdade de Veterinaria, Universidade Federal do Rio Grande do Sul, Rio Grande do Sul, Brazil. carlini@portoweb.com.br., Morrell JM; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden., Nunes MM; Programa de Pos Graduacao em Medicina Veterinaria, Universidade Federal de Vicosa, Minas Gerais, Brazil., Nogueira CE, Curcio BR; Departamento de Clinicas Veterinaria, Faculdade de Veterinaria, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil., Malschitzky E; Programa de Pos Graduacao Medicina Animal: Equinos, Faculdade de Veterinaria, Universidade Federal do Rio Grande do Sul; Curso de Medicina Veterinaria, Universidade Luterana, Rio Grande do Sul, Brazil.
Jazyk:	angličtina
Zdroj:	Cryo letters [Cryo Letters] 2020 Jan-Feb; Vol. 41 (1), pp. 6-12.
Abstrakt:	Background: Epididymal sperm cryopreservation represents the ultimate option to preserve spermatozoa of valuable stallions. Objective: The study aims to evalute whether single layer centrifugation (SLC) prior to cryopreservation or after post-thawing improves the quality of stallion epididymal sperm. Materials and Methods: Epididymal sperms of stallions were harvested (N=20). Sperm samples were subjected to treatments: conventional centrifugation, SLC prior to cryopreservation (SLC-PC) or SLC post-thaw (SLC+). All samples were cryopreserved, thawed and evaluated. SLC+ were thawed, single layer cenrifuged and resuspended in freezing extender (SLC+F) or cooling extender (SLC+C). Total motility, progressive motility, morphology, mitochondrial functionality, membrane integrity and DNA integrity were evaluated. Results: SLC-PC and SLC+F yielded higher total motility, while SLC+F yielded the highest progressive motility. Mitochondrial functionality was significantly higher in all SLC groups. Membrane integrity was higher in SLC-PC. The percentage of morphologically normal spermatozoa was higher in SLC-PC and SLC+F. Conclusion: SLC prior to cryopreservation or post-thaw improves the quality of stallion epididymal spermatozoa. When SLC is performed post-thaw, freezing extender is the best medium to resuspend the pelleted semen.
Databáze:	MEDLINE
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