FERONIA restricts Pseudomonas in the rhizosphere microbiome via regulation of reactive oxygen species.

Autor: Song Y; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada.; Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia, Canada., Wilson AJ; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada., Zhang XC; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA.; Department of Genetics, Harvard Medical School, Boston, MA, USA.; DermBiont, Boston, MA, USA., Thoms D; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada.; Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia, Canada., Sohrabi R; Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI, USA., Song S; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada., Geissmann Q; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada.; Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia, Canada., Liu Y; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada., Walgren L; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada., He SY; Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, MI, USA.; Department of Biology, Duke University, Durham, NC, USA.; Howard Hughes Medical Institute, Duke University, Durham, NC, USA., Haney CH; Department of Microbiology and Immunology, The University of British Columbia, Vancouver, British Columbia, Canada. cara.haney@msl.ubc.ca.; Michael Smith Laboratories, The University of British Columbia, Vancouver, British Columbia, Canada. cara.haney@msl.ubc.ca.
Jazyk: angličtina
Zdroj: Nature plants [Nat Plants] 2021 May; Vol. 7 (5), pp. 644-654. Date of Electronic Publication: 2021 May 10.
DOI: 10.1038/s41477-021-00914-0
Abstrakt: Maintaining microbiome structure is critical for the health of both plants and animals. By re-screening a collection of Arabidopsis mutants affecting root immunity and hormone crosstalk, we identified a FERONIA (FER) receptor kinase mutant (fer-8) with a rhizosphere microbiome enriched in Pseudomonas fluorescens without phylum-level dysbiosis. Using microbiome transplant experiments, we found that the fer-8 microbiome was beneficial. The effect of FER on rhizosphere pseudomonads was largely independent of its immune scaffold function, role in development and jasmonic acid autoimmunity. We found that the fer-8 mutant has reduced basal levels of reactive oxygen species (ROS) in roots and that mutants deficient in NADPH oxidase showed elevated rhizosphere pseudomonads. The addition of RALF23 peptides, a FER ligand, was sufficient to enrich P. fluorescens. This work shows that FER-mediated ROS production regulates levels of beneficial pseudomonads in the rhizosphere microbiome.
Databáze: MEDLINE
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