Evaluation of the Freezability of the Bovine Epididymis Tail Sperm with the Addition of Antioxidants.

Autor:	Passamani da Cruz G; Paranaense University, Umuarama/PR, Brazil., Zanfrilli Dos Santos AP; Paranaense University, Umuarama/PR, Brazil., de Freitas Guaitolini CR; Paranaense University, Umuarama/PR, Brazil., Tramontin MLD; Paranaense University, Umuarama/PR, Brazil., Rigoto RP; Paranaense University, Umuarama/PR, Brazil., Crespilho AM; Santo Amaro University, Sao Paulo/SP, Brazil., Dell'Aqua CPF; Department of Radiology and Animal Reproduction, FMVZ/UNESP, Botucatu/SP, Brazil., Martins MIM; Londrina State University, UEL, Londrina/PR, Brazil., Marques AB; Londrina State University, UEL, Londrina/PR, Brazil., Hidalgo MMT; Londrina State University, UEL, Londrina/PR, Brazil., Sestari DAO; Paranaense University, Umuarama/PR, Brazil., Magalhaes R; Paranaense University, Umuarama/PR, Brazil., Maziero RRD; Paranaense University, Umuarama/PR, Brazil. rosiaramaziero@prof.unipar.br.
Jazyk:	angličtina
Zdroj:	Cryo letters [Cryo Letters] 2021 Mar-Apr; Vol. 42 (2), pp. 81-86.
Abstrakt:	Background: The cryopreservation and recovery of epididymis tail sperm is an important biotechnology dependent on the composition of the freezing medium. Objetive: To evaluate the effect of melatonin, added to commercial freezing medium extender, on the kinetics and viability of bovine epididymis tail sperm. Material and Methods: Five routines were performed, each consisting of eight epididymis and the structures were sliced onto a glass plate containing a commercial diluting medium for Botubov. The samples were divided into four groups, with 80 x 10 6 spermatozoa per mL. Group 1: samples diluted in Botubov. Group 2: samples centrifuged (600 g, 10 min), and the pellet re-suspended in Botubov. Group 3, samples diluted in Botubov containing 100 pM melatonin. Group 4: samples centrifuged (600 g, 10 min) and the pellet resuspended in Botubov with 100 pM melatonin. The samples were transferred to 0.5 mL straws at 40 x 10 6 viable spermatozoa, stabilized at 5º C for 4 h, transferred to liquid nitrogen vapour for 20 min, dipped in liquid nitrogen and stored in a cryogenic cylinder. After thawing (46ºC, 15s), sperm kinetics and viability parameters were evaluated. Results: There was no difference in the parameters of total motility (MT, %), progressive motility (MP, %), progressive linear velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), linearity (LIN, %), spermatozoa with rapid movement (RAP, %) and level of intact plasma membranes and acrosome (IPMA, %) among the groups studied. However, a difference was observed between the routines performed. Conclusion: The protocol for freezing bovine epididymis tail sperm is applicable; however, there is an influence of the epididymis used, for the best efficacy of this biotechnology.
Databáze:	MEDLINE
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