Can Serum be a Match for Urine in the Regulatory Analysis of Boldenone in Cattle? A Systematic Comparison Between Detection Window, Stability, and Enzymatic Hydrolysis.

Autor:	Rocha DG; Department of Chemistry-Institute of Exact Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG31270-901, Brazil.; Federal Laboratory of Animal and Plant Health and Inspection, Ministry of Agriculture, Livestock and Food Supply, Av. Rômulo Joviano s/n, Pedro Leopoldo, MG 33600-000, Brazil., Lana MAG; Federal Laboratory of Animal and Plant Health and Inspection, Ministry of Agriculture, Livestock and Food Supply, Av. Rômulo Joviano s/n, Pedro Leopoldo, MG 33600-000, Brazil., Dracz S; Federal Laboratory of Animal and Plant Health and Inspection, Ministry of Agriculture, Livestock and Food Supply, Av. Rômulo Joviano s/n, Pedro Leopoldo, MG 33600-000, Brazil., da Silva RR; Federal Laboratory of Animal and Plant Health and Inspection, Ministry of Agriculture, Livestock and Food Supply, Av. Rômulo Joviano s/n, Pedro Leopoldo, MG 33600-000, Brazil., de Assis DCS; Veterinary School, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG 30123-970, Brazil., da Silva MC; Department of Chemistry-Institute of Exact Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG31270-901, Brazil., Augusti R; Department of Chemistry-Institute of Exact Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG31270-901, Brazil., Faria AF; Department of Chemistry-Institute of Exact Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG31270-901, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of agricultural and food chemistry [J Agric Food Chem] 2021 May 19; Vol. 69 (19), pp. 5528-5535. Date of Electronic Publication: 2021 May 10.
DOI:	10.1021/acs.jafc.1c00878
Abstrakt:	This work involved a systematic comparison between serum and urine for the monitoring of anabolic androgenic steroids in livestock. Incurred samples were collected over 120 days from crossbred steers treated with intramuscular injections containing boldenone undecylenate. Independent high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) methods were used for the assessment of the respective detection windows, which were larger for serum samples. Both matrices presented adequate performance in terms of long-term stability, assessed using an isochronous approach during 196 days at -20 °C and for five freeze-thaw cycles. The effectiveness of the enzymatic hydrolysis reaction using Helix pomatia juice was also compared. The calculated concentrations in serum samples were not statistically influenced by the deconjugation reaction. On the other hand, urine hydrolysis conditions were studied using a 3 3 Box-Behnken Design, in which a central point condition led to a satisfactory deconjugation performance. It could be observed that serum exhibited equivalent or better performance than urine for most of the evaluated criteria; thus, its inclusion in the regulatory analysis of boldenone in cattle is supported.
Databáze:	MEDLINE
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