Area from image analyses accurately estimates dry-weight biomass of juvenile tissue from the moss Ceratodon purpureus .

Autor: Burtscher WP; Department of Biology University of Florida Gainesville Florida 32611 USA., List MA; Department of Biology University of Florida Gainesville Florida 32611 USA., Payton AC; Department of Biology University of Florida Gainesville Florida 32611 USA., McDaniel SF; Department of Biology University of Florida Gainesville Florida 32611 USA., Carey SB; Department of Biology University of Florida Gainesville Florida 32611 USA.
Jazyk: angličtina
Zdroj: Applications in plant sciences [Appl Plant Sci] 2021 Apr 30; Vol. 9 (4), pp. e11418. Date of Electronic Publication: 2021 Apr 30 (Print Publication: 2021).
DOI: 10.1002/aps3.11418
Abstrakt: Premise: Mosses have long served as models for studying many areas of plant biology. Investigators have used two-dimensional measurements of juvenile growth from photographs as a surrogate for dry-weight biomass. The relationship between area and biomass, however, has not been critically evaluated.
Methods: Here we grew axenic tissue cultures of 10 Ceratodon purpureus isolates to study the relationship between these parameters. We measured area and biomass on replicate cultures with two distinct starting inoculum sizes each week for three weeks. We then examined the correlation between area and biomass as well as the influence of variation in inoculum size on both parameters.
Results: We found a strong correlation between area and biomass after two weeks of growth. Furthermore, we found inoculum size affected biomass during the first week of growth but not in subsequent weeks and inoculum size had no detectable effect on area.
Discussion: These analyses provide experimental confirmation that area is a suitable proxy for biomass and provide clear guidelines for when inoculum size variation may affect downstream growth estimates.
(© 2021 Burtscher et al. Applications in Plant Sciences is published by Wiley Periodicals LLC on behalf of the Botanical Society of America.)
Databáze: MEDLINE
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