Phytochemical profiling of several Hypericum species identified using genetic markers.

Autor: Bruňáková K; Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia. Electronic address: katarina.brunakova@upjs.sk., Bálintová M; Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia. Electronic address: miroslava.balintova@upjs.sk., Henzelyová J; Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia. Electronic address: jana.henzelyova@upjs.sk., Kolarčik V; Department of Botany, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia. Electronic address: vladislav.kolarcik@upjs.sk., Kimáková A; Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia; Present Address: Department of Epizootiology and Parasitology, Institute of Parasitology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181, Košice, Slovakia. Electronic address: andrea.kimakova@uvlf.sk., Petijová L; Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia. Electronic address: linda.petijova@upjs.sk., Čellárová E; Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia. Electronic address: eva.cellarova@upjs.sk.
Jazyk: angličtina
Zdroj: Phytochemistry [Phytochemistry] 2021 Jul; Vol. 187, pp. 112742. Date of Electronic Publication: 2021 May 06.
DOI: 10.1016/j.phytochem.2021.112742
Abstrakt: In the present study, we performed phytochemical profiling of several under-exploited Hypericum representatives taxonomically belonging to the sections Ascyreia, Androsaemum, Inodora, Hypericum, Coridium, Myriandra, and Adenosepalum. The authenticity of the starting plant material was confirmed using the nuclear ribosomal internal transcribed spacer as a molecular marker, DNA content and chromosome number. Phenolic constituents were analyzed using high-performance liquid chromatography to complement species-specific metabolic profiles. In several Hypericum representatives, the pharmacologically important compounds, including naphthodianthrones; phloroglucinol derivatives; chlorogenic acid; and some classes of flavonoids, particularly the flavonols rutin and hyperoside, flavanol catechin, and flavanones naringenin and naringin, were reported for the first time. Comparative multivariate analysis of chemometric data for seedlings cultured in vitro and acclimated to the outdoor conditions revealed a strong genetically predetermined interspecific variability in phenolic compound content. In addition to hypericins, which are the most abundant chemomarkers for the genus Hypericum, rarely employed phenolic metabolites, including phloroglucinol derivatives, chlorogenic acid, catechin, naringenin, naringin, and kaempferol-3-O-glucoside, were shown to be useful for discriminating between closely related species. Given the increasing interest in natural products of the genus Hypericum, knowledge of the spectrum of phenolic compounds in shoot cultures is a prerequisite for future biotechnological applications. In addition, phytochemical profiling should be considered as an additional part of the integrated plant authentication system, which predominantly relies upon genetic markers.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: