Antitumor and antimigration effects of Salvia clandestina L. extract on osteosarcoma cells.

Autor: Muscella A; Department of Biological and Environmental Science and Technologies (DiSTeBA), University of Salento, Lecce, Italy., Stefàno E; Department of Biological and Environmental Science and Technologies (DiSTeBA), University of Salento, Lecce, Italy., De Bellis L; Department of Biological and Environmental Science and Technologies (DiSTeBA), University of Salento, Lecce, Italy., Nutricati E; Department of Biological and Environmental Science and Technologies (DiSTeBA), University of Salento, Lecce, Italy., Negro C; Department of Biological and Environmental Science and Technologies (DiSTeBA), University of Salento, Lecce, Italy., Marsigliante S; Department of Biological and Environmental Science and Technologies (DiSTeBA), University of Salento, Lecce, Italy.
Jazyk: angličtina
Zdroj: Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 2021 Sep; Vol. 1500 (1), pp. 34-47. Date of Electronic Publication: 2021 May 07.
DOI: 10.1111/nyas.14601
Abstrakt: Salvia clandestina L. is a wild perennial species present in the Salento area of Italy. Here, we examined the in vitro effects of an aqueous extract of S. clandestina L. on the MG-63 osteosarcoma cell line. The extract reduced osteosarcoma cell viability mainly by way of apoptosis, as we observed (1) upregulation of gene and protein expression of p53, cyclin-dependent kinase inhibitors p21 WAF1 and p27 Kip1 , and proapoptotic BAX; (2) activation of caspases; and (3) induction of a sub-G 1 peak in the cell cycle. The mitogen-activated protein kinases (MAPKs) JNK1/2 and p38 are activated and involved in the intracellular effects of the S. clandestina extract, as preincubation with the JNK1/2 inhibitor SP600125 or the p38 inhibitor SB203580 significantly decreased S. clandestina extract-induced cytotoxicity and inhibited increase in p53, p21 WAF1 , p27 Kip1 , and BAX. SP600125 also inhibited mRNA levels for all the aforementioned proteins, while SB203580 only affected p53 mRNA. Furthermore, S. clandestina extract treatment counteracted epithelial-to-mesenchymal transition, inhibited cell migration, and decreased the expression and activity of matrix metalloproteinase MMP2. In addition, S. clandestina extract enhanced the cytotoxic activity of cisplatin on MG-63 cells through downregulation of the Akt/PKB protein kinase. We conclude that S. clandestina extract may be a novel agent for osteosarcoma treatment.
(© 2021 The Authors. Annals of the New York Academy of Sciences published by Wiley Periodicals LLC on behalf of New York Academy of Sciences.)
Databáze: MEDLINE
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