Production of Secreted Carbohydrates that Present Immunologic Similarities with the Cryptococcus Glucuronoxylomannan by Members of the Trichosporonaceae Family: A Comparative Study Among Species of Clinical Interest.

Autor: de Andrade IB; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil.; Laboratório de Biofísica de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Almeida MA; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil., Figueiredo-Carvalho MHG; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil., Coelho RA; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil., Chaves ALDS; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil.; Laboratório de Patologia Clínica, Instituto Nacional de Câncer, Rio de Janeiro, RJ, Brazil., Frases S; Laboratório de Biofísica de Fungos, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Zancopé-Oliveira RM; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil., Brito-Santos F; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil., Almeida-Paes R; Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brazil 4365-Manguinhos, Fiocruz, Rio de Janeiro, RJ, 21045-900, Brazil. rodrigo.paes@ini.fiocruz.br.
Jazyk: angličtina
Zdroj: Mycopathologia [Mycopathologia] 2021 Jun; Vol. 186 (3), pp. 377-385. Date of Electronic Publication: 2021 May 06.
DOI: 10.1007/s11046-021-00558-w
Abstrakt: Glucuronoxylomannan (GXM) participates in several immunoregulatory mechanisms, which makes it an important Cryptococcus virulence factor that is essential for the disease. Trichosporon asahii and Trichosporon mucoides share with Cryptococcus species the ability to produce GXM. To check whether other opportunistic species in the Trichosporonaceae family produce GXM-like polysaccharides, extracts from 28 strains were produced from solid cultures and their carbohydrate content evaluated by the sulfuric acid / phenol method. Moreover, extracts were assessed for cryptococcal GXM cross-reactivity through latex agglutination and lateral flow assay methods. Cryptococcus neoformans and Saccharomyces cerevisiae were used as positive and negative controls, respectively. In addition to T. asahii, the species Trichosporon inkin, Apiotrichum montevideense, Trichosporon japonicum, Trichosporon faecale, Trichosporon ovoides, Cutaneotrichosporon debeurmannianum, and Cutaneotrichosporon arboriformis are also producers of a polysaccharide immunologically similar to the GXM produced by human pathogenic Cryptococcus species. The carbohydrate concentration of the extracts presented a positive correlation with the GXM contents determined by titration of both methodologies. These results add several species to the list of fungal pathogens that produce glycans of the GXM type and bring information about the origin of potential false-positive results on immunological tests for diagnosis of cryptococcosis based on GXM detection.
Databáze: MEDLINE
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