| Autor: |
Meccatti VM; Universidade Estadual Paulista(UNESP), Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, Av. Engenheiro Francisco José Longo, 777, 12245-000 São José dos Campos, SP, Brazil., Oliveira JR; Universidade Anhembi Morumbi, Escola de Medicina, Av. Dep. Benedito Matarazzo, 4050, 12230-002 São José dos Campos, SP, Brazil., Figueira LW; Universidade Estadual Paulista(UNESP), Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, Av. Engenheiro Francisco José Longo, 777, 12245-000 São José dos Campos, SP, Brazil., Lagareiro Netto AA; Universidade Anhanguera, Departamento de Farmácia, Av. Raimundo Pereira de Magalhães, 3305, 05145-200 São Paulo, SP, Brazil., Zamarioli LS; Universidade Federal de São Paulo(UNIFESP), Instituto de Farmacologia e Biologia Molecular, Departamento de Modo de Ação de Drogas, Rua Três de Maio, 100, 04044-020 São Paulo, SP, Brazil., Marcucci MC; Universidade Estadual Paulista(UNESP), Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, Av. Engenheiro Francisco José Longo, 777, 12245-000 São José dos Campos, SP, Brazil., Camargo SEA; University of Florida, College of Dentistry, Department of Restorative Dental Sciences, Gainesville, FL, 32610, USA., Carvalho CAT; Universidade Estadual Paulista(UNESP), Instituto de Ciência e Tecnologia, Departamento de Odontologia Restauradora, Av. Engenheiro Francisco José Longo, 777, 12245-000 São José dos Campos, SP, Brazil., Oliveira LD; Universidade Estadual Paulista(UNESP), Instituto de Ciência e Tecnologia, Departamento de Biociências e Diagnóstico Bucal, Av. Engenheiro Francisco José Longo, 777, 12245-000 São José dos Campos, SP, Brazil. |
| Abstrakt: |
Candida spp. are naturally opportunistic and can promote infections. These yeasts can form biofilm, after penetration and adhesion to the biotic or abiotic surfaces. Preexisting diseases, treatments with drugs and radiation therapy, medical procedures, and parafunctional habits favor the installation of a fungal infection. Increased resistance to the available antifungals has become a concern. Therefore, alternative methods to control them have been evaluated, including the use of plant substances. In this study, the antibiofilm effect of R. officinalis L. extract was analyzed on C. albicans, C. dubliniensis, C. glabrata, C. krusei, and C. tropicalis. A phytochemical analysis of the extract was performed. Biofilms were formed for 48 h and exposed to the different concentrations of the extract (50, 100, and 200 mg/mL) for 5 min or 24 h. The effect of the plant extract was compared to the antifungal nystatin. Rosmarinus officinalis L. extract was constituted of phenols and flavonoids, highlighting the presence of chlorogenic acid derivatives in its composition. Biofilm reductions were observed after exposure to the plant extract for both periods. The plant extract provided a reduction similar to the antifungal. Thus, R. officinalis L. extract showed antibiofilm effect on Candida spp. comparable to the nystatin. |
