Anthelmintic resistance of Haemonchus contortus from sheep flocks in Brazil: concordance of in vivo and in vitro (RESISTA-Test©) methods.

Autor: Gainza YA; Faculdade de Ciências Agrárias e Veterinárias - FCAV, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil., Santos IBD; Faculdade de Ciências Agrárias e Veterinárias - FCAV, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil., Figueiredo A; Faculdade de Ciências Agrárias e Veterinárias - FCAV, Universidade Estadual Paulista - UNESP, Jaboticabal, SP, Brasil., Santos LALD; Centro Universitário Central Paulista - UNICEP, São Carlos, SP, Brasil., Esteves SN; Embrapa Pecuária Sudeste - CPPSE, São Carlos, SP, Brasil., Barioni-Junior W; Embrapa Pecuária Sudeste - CPPSE, São Carlos, SP, Brasil., Minho AP; Embrapa Pecuária Sudeste - CPPSE, São Carlos, SP, Brasil., Chagas ACS; Embrapa Pecuária Sudeste - CPPSE, São Carlos, SP, Brasil.
Jazyk: angličtina
Zdroj: Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria [Rev Bras Parasitol Vet] 2021 May 03; Vol. 30 (2), pp. e025120. Date of Electronic Publication: 2021 May 03 (Print Publication: 2021).
DOI: 10.1590/S1984-296120201093
Abstrakt: This study evaluated the resistance status of Haemonchus contortus from sheep flocks in the state of São Paulo, Brazil, through comparison between the fecal egg count reduction test (FECRT) and the larval development test (LDT). For the FECRT, 35 sheep were selected in each of five flocks and divided into groups treated with: benzimidazole, levamisole, ivermectin, monepantel and control. Feces were collected for EPG and fecal cultures. The LDT was performed using thiabendazole (TBZ), levamisole (LEV), ivermectin aglycone (IVM-A) and Zolvix (ZLV). Resistance to all drugs was detected using FECRT in 100% of the flocks, except in relation to ZLV (40% resistant and 20% suspected of resistance). LDT indicated resistance to TBZ and IVM-A in all flocks, to LEV in 80% of flocks and to ZLV in 10%. Total agreement was obtained between the two tests for TBZ and IVM (k = 1.0), while for LEV (k = 0.8) and ZLV (k = 0.9), substantial and almost perfect agreement were obtained, respectively. The concordance between the tests was significant, thus showing that it is possible to use the outcome of the LDT to predict the FECRT, and hence validating the former as a fast diagnostic test for use by sheep farmers in Brazil.
Databáze: MEDLINE