| Autor: |
Parussolo L; Universidade do Estado de Santa Catarina, Centro de Diagnóstico Microbiológico Animal, Centro de Ciências Agroveterinárias, Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil.; Instituto Federal de Santa Catarina, Av. Mauro Ramos, 950, Centro, 88020-300 Florianópolis, SC, Brazil., Sfaciotte RAP; Universidade do Estado de Santa Catarina, Centro de Diagnóstico Microbiológico Animal, Centro de Ciências Agroveterinárias, Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil., Dalmina KA; Universidade do Estado de Santa Catarina, Centro de Diagnóstico Microbiológico Animal, Centro de Ciências Agroveterinárias, Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil., Melo FD; Universidade do Estado de Santa Catarina, Centro de Diagnóstico Microbiológico Animal, Centro de Ciências Agroveterinárias, Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil., Costa UMD; Universidade do Estado de Santa Catarina, Centro de Diagnóstico Microbiológico Animal, Centro de Ciências Agroveterinárias, Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil., Ferraz SM; Universidade do Estado de Santa Catarina, Centro de Diagnóstico Microbiológico Animal, Centro de Ciências Agroveterinárias, Av. Luiz de Camões, 2090, Bairro Conta Dinheiro, 88520-000 Lages, SC, Brazil. |
| Abstrakt: |
Listeria monocytogenes is an opportunistic pathogen that causes listeriosis, a foodborne disease with low incidence but with high mortality rate in humans. This microorganism has been recovered from several dairy products, especially those produced with raw milk. The objective of this work was to investigate the presence of virulence genes, and also to define the antimicrobial susceptibility profile of L. monocytogenes isolates recovered from serrano artisanal cheese produced in Southern region of Brazil. Nine strains of L. monocytogenes (serotypes 1/2b and 4b) were evaluated through PCR to detect the presence of the virulence genes hly, inlA, inlC, inlJ, actA, plcB and iap, while antimicrobial susceptibility profile was determined via disk diffusion method. All strains exhibited the presence of the genes hly and plcB, whereas the other genes (iap, actA, inlA, inlC and inlJ) were only detected in eight strains. We verified that all strains were resistant to at least one antimicrobial agent and three of them showed multidrug resistance. These findings demonstrated the serrano artisanal cheese offers risks to consumers' health and point to a need of adaptations and monitoring of manufacturing process of this food, in order to prevent the dissemination of L. monocytogenes. |
