| Autor: |
Ito M; Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan., Toriumi T; Department of Oral Anatomy, School of Dentistry, Aichi Gakuin University, Aichi 464-8650, Japan., Hiratsuka T; Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan., Imura H; Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan., Akiyama Y; Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan., Chimedtseren I; Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan., Arai Y; Department of Oral and Maxillofacial Radiology, Nihon University School of Dentistry, Tokyo 101-8310, Japan., Yamaguchi K; Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan., Azuma A; Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan., Hata KI; Bio Science & Engineering Laboratory, Research & Development Management Headquarters FUJIFILM Corporation, Kanagawa 258-8577, Japan., Natsume N; Division of Research and Treatment for Oral and Maxillofacial Congenital Anomalies, School of Dentistry, Aichi Gakuin University, Aichi 464-8651, Japan., Honda M; Department of Oral Anatomy, School of Dentistry, Aichi Gakuin University, Aichi 464-8650, Japan. |
| Abstrakt: |
This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration of the heat-dependent dehydration reaction. Rat palatine fissures ( n = 45), analogous to human congenital bone defects, were examined to evaluate the potential of bone formation by the three different RCP particles. Microcomputed tomography images were obtained to measure bone volume and bone mineral density at 4, 8, 12, and 16 weeks post grafting. Specimens were obtained for histological analysis at 16 weeks after grafting. Additionally, alkaline phosphatase and tartrate acid phosphatase staining were performed to visualize the presence of osteoblasts and osteoclasts. At 16 weeks, bone volume, bone mineral density, and new bone area measurements in group 2 were significantly higher than in any other group. In addition, the number of osteoblasts and osteoclasts on the new bone surface in group 2 was significantly higher than in any other group. Our results demonstrated that medium cross-linking was more suitable for bone formation-and could be useful in human alveolar cleft repairs as well. |
