Exploring the diversity and hydrocarbon bioremediation potential of microbial community in the waste sludge of Duliajan oil field, Assam, India.

Autor: Pal S; Department of Biotechnology, National Institute of Technology Durgapur, Durgapur, WB, 713209, India., Dutta A; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, WB, 721302, India.; School of Bio Science, Indian Institute of Technology Kharagpur, Kharagpur, WB, 721302, India., Sarkar J; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, WB, 721302, India., Roy A; Department of Biotechnology, National Institute of Technology Durgapur, Durgapur, WB, 713209, India., Sar P; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, WB, 721302, India., Kazy SK; Department of Biotechnology, National Institute of Technology Durgapur, Durgapur, WB, 713209, India. sufia_kazy@yahoo.com.
Jazyk: angličtina
Zdroj: Environmental science and pollution research international [Environ Sci Pollut Res Int] 2021 Sep; Vol. 28 (36), pp. 50074-50093. Date of Electronic Publication: 2021 May 04.
DOI: 10.1007/s11356-021-13744-6
Abstrakt: Microbial community analysis of crude oil containing sludge collected from Duliajan oil field, Assam, India, showed the predominance of hydrocarbon-degrading bacteria such as Pseudomonas (20.1%), Pseudoxanthomonas (15.8%), Brevundimonas (1.6%), and Bacillus (0.8%) alongwith anaerobic, fermentative, nitrogen-fixing, nitrate-, sulfate-, and metal-reducing, syntrophic bacteria, and methanogenic archaea. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated gene collection for potential hydrocarbon degradation, lipid, nitrogen, sulfur, and methane metabolism. The culturable microbial community was predominated by Pseudomonas and Bacillus with the metabolic potential for utilizing diverse hydrocarbons, crude oil, and actual petroleum sludge as sole carbon source during growth and tolerating various environmental stresses prevailing in such contaminated sites. More than 90% of the isolated strains could produce biosurfactant and exhibit catechol 2,3-dioxygenase activity. Nearly 30% of the isolates showed alkane hydroxylase activity with the maximum specific activity of 0.54 μmol min -1 mg -1 . The study provided better insights into the microbial diversity and functional potential within the crude oil containing sludge which could be exploited for in situ bioremediation of contaminated sites.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
Externí odkaz: