| Autor: |
De la Rubia AG; Área de Fisiología Vegetal, Departamento de Ingeniería y Ciencias Agrarias, Universidad de León, E-24071, León, Spain., Centeno ML; Área de Fisiología Vegetal, Departamento de Ingeniería y Ciencias Agrarias, Universidad de León, E-24071, León, Spain., Moreno-González V; Área de Zoología, Departamento de Biodiversidad y Gestión Ambiental, Universidad de León, E-24071, León, Spain., De Castro M; Departamento de Biotecnología Vegetal, Laboratorios Analíticos Agrovet, Mansilla Mayor, 24217, León, España., García-Angulo P; Área de Fisiología Vegetal, Departamento de Ingeniería y Ciencias Agrarias, Universidad de León, E-24071, León, Spain. |
| Jazyk: |
angličtina |
| Zdroj: |
Phytopathology [Phytopathology] 2021 Dec; Vol. 111 (12), pp. 2332-2342. Date of Electronic Publication: 2021 Dec 13. |
| DOI: |
10.1094/PHYTO-10-20-0449-R |
| Abstrakt: |
Common bean ( Phaseolus vulgaris ) is attacked by several pathogens such as the biotrophic gamma-proteobacterium Pseudomonas syringae pv. phaseolicola . To study the P. syringae pv. phaseolicola -bean interaction during the first stages of infection, leaf discs of a susceptible bean cultivar Riñón were infected with pathogenic P. syringae pv. phaseolicola . Using this experimental system, we tested six new putative wall-associated kinase (WAK) receptors, previously identified in silico. These six P. vulgaris WAKs (PvWAKs) showed high protein sequence homology to the well-described Arabidopsis thaliana WAK1 (AtWAK1) receptor and, by phylogenetic analysis, clustered together with AtWAKs. The expression of PvWAK 1 increased at very early stages after the P. syringae pv. phaseolicola infection. Time course experiments were performed to evaluate the accumulation of apoplastic H 2 O 2 , Ca 2+ influx, total H 2 O 2 , antioxidant enzymatic activities, lipid peroxidation, and the concentrations of abscisic acid and salicylic acid (SA), as well as the expression of six defense-related genes: MEKK-1 , MAPKK , WRKY33 , RIN4 , PR1 , and NPR1 . The results showed that overexpression of PR1 occurred 2 h after P. syringae pv. phaseolicola infection without a concomitant increase in SA levels. Although apoplastic H 2 O 2 increased after infection, the oxidative burst was neither intense nor rapid, and an efficient antioxidant response did not occur, suggesting that the observed cellular damage was caused by the initial increase in total H 2 O 2 early after infection. In conclusion, Riñón can perceive the presence of P. syringae pv. phaseolicola , but this recognition results in only a modest and slow activation of host defenses, leading to high susceptibility to P. syringae pv. phaseolicola . |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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