Multiple lineages of Streptomyces produce antimicrobials within passalid beetle galleries across eastern North America.

Autor:	Pessotti RC; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States., Hansen BL; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States., Reaso JN; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States., Ceja-Navarro JA; Bioengineering and Biomedical Sciences Department, Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, United States.; Institute for Biodiversity Science and Sustainability, California Academy of Sciences, Berkeley, United States., El-Hifnawi L; Department of Molecular and Cellular Biology, University of California, Berkeley, Berkeley, United States., Brodie EL; Ecology Department, Earth and Environmental Sciences, Lawrence Berkeley National Laboratory, Berkeley, United States.; Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, United States., Traxler MF; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 May 04; Vol. 10. Date of Electronic Publication: 2021 May 04.
DOI:	10.7554/eLife.65091
Abstrakt:	Some insects form symbioses in which actinomycetes provide defense against pathogens by making antimicrobials. The range of chemical strategies employed across these associations, and how these strategies relate to insect lifestyle, remains underexplored. We assessed subsocial passalid beetles of the species Odontotaenius disjunctus , and their frass (fecal material), which is an important food resource within their galleries, as a model insect/actinomycete system. Through chemical and phylogenetic analyses, we found that O. disjunctus frass collected across eastern North America harbored multiple lineages of Streptomyces and diverse antimicrobials. Metabolites detected in frass displayed synergistic and antagonistic inhibition of a fungal entomopathogen, Metarhizium anisopliae , and multiple streptomycete isolates inhibited this pathogen when co-cultivated directly in frass. These findings support a model in which the lifestyle of O. disjunctus accommodates multiple Streptomyces lineages in their frass, resulting in a rich repertoire of antimicrobials that likely insulates their galleries against pathogenic invasion. Competing Interests: RP, BH, JR, JC, LE, EB, MT No competing interests declared
Databáze:	MEDLINE
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