Compliant 3D frameworks instrumented with strain sensors for characterization of millimeter-scale engineered muscle tissues.

Autor:	Zhao H; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208.; Department of Aerospace and Mechanical Engineering, University of Southern California, Los Angeles, CA 90089., Kim Y; Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801.; Nick J. Holonyak Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Wang H; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208.; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208.; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208., Ning X; Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Xu C; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208., Suh J; Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Han M; Department of Biomedical Engineering, College of Future Technology, Peking University, 100871 Beijing, China., Pagan-Diaz GJ; Nick J. Holonyak Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801.; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Lu W; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208., Li H; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208.; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208.; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208., Bai W; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208., Aydin O; Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Park Y; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208., Wang J; Nick J. Holonyak Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801.; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Yao Y; Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801., He Y; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208.; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208.; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208., Saif MTA; Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801., Huang Y; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208; y-huang@northwestern.edu rbashir@illinois.edu jrogers@northwestern.edu.; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208.; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208.; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208., Bashir R; Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801; y-huang@northwestern.edu rbashir@illinois.edu jrogers@northwestern.edu.; Nick J. Holonyak Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801.; Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801.; Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801.; Department of Biomedical and Translational Sciences, Carle Illinois College of Medicine, Urbana, IL 61801., Rogers JA; Querrey Simpson Institute for Bioelectronics, Northwestern University, Evanston, IL 60208; y-huang@northwestern.edu rbashir@illinois.edu jrogers@northwestern.edu.; Department of Materials Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801.; Department of Materials Science and Engineering, Northwestern University, Evanston, IL 60208.; Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL 60208.; Department of Mechanical Engineering, Northwestern University, Evanston, IL 60208.; Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208.; Department of Neurological Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL 60611.; Department of Chemistry, Northwestern University, Evanston, IL 60208.; Department of Electrical Engineering and Computer Science, Northwestern University, Evanston, IL 60208.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 May 11; Vol. 118 (19).
DOI:	10.1073/pnas.2100077118
Abstrakt:	Tissue-on-chip systems represent promising platforms for monitoring and controlling tissue functions in vitro for various purposes in biomedical research. The two-dimensional (2D) layouts of these constructs constrain the types of interactions that can be studied and limit their relevance to three-dimensional (3D) tissues. The development of 3D electronic scaffolds and microphysiological devices with geometries and functions tailored to realistic 3D tissues has the potential to create important possibilities in advanced sensing and control. This study presents classes of compliant 3D frameworks that incorporate microscale strain sensors for high-sensitivity measurements of contractile forces of engineered optogenetic muscle tissue rings, supported by quantitative simulations. Compared with traditional approaches based on optical microscopy, these 3D mechanical frameworks and sensing systems can measure not only motions but also contractile forces with high accuracy and high temporal resolution. Results of active tension force measurements of engineered muscle rings under different stimulation conditions in long-term monitoring settings for over 5 wk and in response to various chemical and drug doses demonstrate the utility of such platforms in sensing and modulation of muscle and other tissues. Possibilities for applications range from drug screening and disease modeling to biohybrid robotic engineering. Competing Interests: The authors declare no competing interest.
Databáze:	MEDLINE
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