Inhibition of Bemisia tabaci vectored, GroEL mediated transmission of tomato leaf curl New Delhi virus by garlic leaf lectin (Allium sativum leaf agglutinin).

Autor: Das A; Division of Plant Biology, Bose Institute, P1/12, C. I. T Scheme VIIM, Kolkata, 700054, West Bengal, India; Department of Life Sciences, Presidency University, 86/1 College Street, Kolkata, 700073, India(1). Electronic address: ayan.dbs@presiuniv.ac.in., Roy A; Division of Plant Biology, Bose Institute, P1/12, C. I. T Scheme VIIM, Kolkata, 700054, West Bengal, India; Czech University of Life Sciences Prague, Faculty of Forestry and Wood Sciences, Excellent Team for Mitigation (ETM), Kamýcká 129, CZ - 165 00 Praha 6 - Suchdol, Czech Republic(1). Electronic address: Roy@fld.czu.cz., Mandal A; Division of Plant Biology, Bose Institute, P1/12, C. I. T Scheme VIIM, Kolkata, 700054, West Bengal, India; Department of Genetics, University of Calcutta, Tarknath Palit Siksha Prangan, UCSTA, 35, Ballygunge Circular Road, Kolkata, 700019, India(1). Electronic address: arunavama@gmail.com., Mondal HA; Division of Plant Biology, Bose Institute, P1/12, C. I. T Scheme VIIM, Kolkata, 700054, West Bengal, India; School of Crop Improvement, College of Post Graduate Studies in Agricultural Sciences, Umiam, Meghalaya, 793103, India(1). Electronic address: hossainalimondal@gmail.com., Hess D; The Protein Analysis Facility, Friedrich Miescher Institute for Biomedical Research Maulbeerstr. 66, 4058, Basel, Switzerland. Electronic address: Daniel.Hess@fmi.ch., Kundu P; Division of Plant Biology, Bose Institute, P1/12, C. I. T Scheme VIIM, Kolkata, 700054, West Bengal, India. Electronic address: pkundu@jcbose.ac.in., Das S; Division of Plant Biology, Bose Institute, P1/12, C. I. T Scheme VIIM, Kolkata, 700054, West Bengal, India. Electronic address: sampa@jcbose.ac.in.
Jazyk: angličtina
Zdroj: Virus research [Virus Res] 2021 Jul 15; Vol. 300, pp. 198443. Date of Electronic Publication: 2021 Apr 30.
DOI: 10.1016/j.virusres.2021.198443
Abstrakt: GroEL or symbionin synthesized by the endosymbionts of whitefly (Bemisia tabaci)/ aphids play a cardinal role in the persistent, circulative transmission of plant viruses by binding to viral coat protein/ read-through protein. Allium sativum leaf agglutinin (ASAL), a Galanthus nivalis agglutinin (GNA)- related mannose-binding lectin from garlic leaf has been reported as a potent controlling agent against hemipteran insects including whitefly and aphids. GroEL related chaperonin- symbionin was previously identified as a receptor of ASAL by the present group in the brush border membrane vesicle (BBMV) of mustard aphid. In the present study similar GroEL receptor of ASAL has been identified through LC-MS/MS in the BBMV of B. tabaci which serves as a vector for several plant viruses including tomato leaf curl New Delhi virus (ToLCNDV). Ligand blot analysis of ASAL-fed B. tabaci showed that when GroEL is pre-occupied by ASAL, it completely blocks its further binding to ToLCNDV coat protein (ToLCNDV-CP). Prior feeding of ASAL hindered the co-localization of ToLCNDV-CP and GroEL in the midgut of B. tabaci. Immunoprecipitation followed by western blot with ASAL-fed B. tabaci yielded similar result. Moreover, ASAL feeding inhibited viral transmission by B. tabaci. Together, these results confirmed that the interaction of ASAL with GroEL interferes with the binding of ToLCNDV-CP and inhibits further B. tabaci mediated viral transmission.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: